ArticleGenetics1997
A genetic strategy to demonstrate the occurrence of spontaneous mutations in nondividing cells within colonies of Escherichia coli.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- A Framework for Investigating Rules of Life by Establishing Zones of Influence.Integrative and comparative biology · 2022Article
- Stress-Induced Mutagenesis.EcoSal Plus · 2012Article
- The rate of mutation of a single gene.Nucleic acids research · 2010Article
- Methods for determining spontaneous mutation rates.Methods in enzymology · 2006Article
- Article
- Mutations arise independently of transcription in non-dividing bacteria.Molecular genetics and genomics : MGG · 2003Article
- lon incompatibility associated with mutations causing SOS induction: null uvrD alleles induce an SOS response in Escherichia coli.Journal of bacteriology · 2000Article
- Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli.Journal of bacteriology · 2000Article
- Adaptive mutation in Escherichia coli.Cold Spring Harbor symposia on quantitative biology · 2000Review
- Determining mutation rates in bacterial populations.Methods (San Diego, Calif.) · 2000Review
- Sorting out mutation rates.Proceedings of the National Academy of Sciences of the United States of America · 1999Review
- Mechanisms of mutation in nondividing cells. Insights from the study of adaptive mutation in Escherichia coli.Annals of the New York Academy of Sciences · 1999Review
- Are adaptive mutations due to a decline in mismatch repair? The evidence is lacking.Mutation research · 1999Review
- Mechanisms of stationary phase mutation: a decade of adaptive mutation.Annual review of genetics · 1999Review
- Adaptive mutation: has the unicorn landed?Genetics · 1998Review
- Stress-induced mutagenesis in bacteria.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A genetic strategy was designed to examine the occurrence of mutations in stationary-phase populations. In this strategy, a parental population of cells is able to survive under both permissive and restrictive conditions whereas mutants at a particular target locus exhibit a conditional-lethal phenotype. Thus, by growing the population to stationary phase under restrictive conditions and then shifting it to permissive conditions, mutations that had arisen in stationary phase can be studied without confounding effects caused by the occurrence of similar mutations during growth of the population. In two different applications of this strategy, we have studied the reversion to Lac+ in stationary phase of several Lac- mutations in Escherichia coli. Our results indicate that a variety of spontaneous point mutations and deletions, particularly those that are sensitive to the mechanisms of replication slippage (for their generation) and methyl-directed mismatch repair (for their correction), can arise in nondividing populations of cells within a colony. The frequency of their occurrence was also elevated in mutS strains, which are defective in such mismatch repair. These data have relevance to the ongoing debate on adaptive or directed mutations in bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.