ArticleThe Journal of cell biology1997
Capping protein terminates but does not initiate chemoattractant-induced actin assembly in Dictyostelium.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- CPI motif interaction is necessary for capping protein function in cells.Nature communications · 2015Article
- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale.Nature communications · 2014Article
- The chemotactic defect in wiskott-Aldrich syndrome macrophages is due to the reduced persistence of directional protrusions.PloS one · 2012Article
- The signaling adaptor Eps8 is an essential actin capping protein for dendritic cell migration.Immunity · 2011Article
- Article
- New insights into mechanism and regulation of actin capping protein.International review of cell and molecular biology · 2008Review
- Structure/function analysis of the interaction of phosphatidylinositol 4,5-bisphosphate with actin-capping protein: implications for how capping protein binds the actin filament.The Journal of biological chemistry · 2007Article
- Phospholipase C and cofilin are required for carcinoma cell directionality in response to EGF stimulation.The Journal of cell biology · 2004Article
- Villin enhances hepatocyte growth factor-induced actin cytoskeleton remodeling in epithelial cells.Molecular biology of the cell · 2003Article
- The Dictyostelium CARMIL protein links capping protein and the Arp2/3 complex to type I myosins through their SH3 domains.The Journal of cell biology · 2001Article
- Phosphatidylinositol 4,5-bisphosphate induces actin stress-fiber formation and inhibits membrane ruffling in CV1 cells.The Journal of cell biology · 2001Article
- Protein tyrosine phosphatase phi regulates paxillin tyrosine phosphorylation and mediates colony-stimulating factor 1-induced morphological changes in macrophages.Molecular and cellular biology · 2001Article
- Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion.The Journal of cell biology · 2000Article
- Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes.The EMBO journal · 2000Article
- Signaling to actin dynamics.The Journal of cell biology · 1999Review
- Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia.The Journal of cell biology · 1999Article
- UNC-60B, an ADF/cofilin family protein, is required for proper assembly of actin into myofibrils in Caenorhabditis elegans body wall muscle.The Journal of cell biology · 1999Article
- Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation.The Journal of cell biology · 1999Article
- Phototactic migration of Dictyostelium cells is linked to a new type of gelsolin-related protein.Molecular biology of the cell · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The first step in the directed movement of cells toward a chemotactic source involves the extension of pseudopods initiated by the focal nucleation and polymerization of actin at the leading edge of the cell. We have previously isolated a chemoattractant-regulated barbed-end capping activity from Dictyostelium that is uniquely associated with capping protein, also known as cap32/34. Although uncapping of barbed ends by capping protein has been proposed as a mechanism for the generation of free barbed ends after stimulation, in vitro and in situ analysis of the association of capping protein with the actin cytoskeleton after stimulation reveals that capping protein enters, but does not exit, the cytoskeleton during the initiation of actin polymerization. Increased association of capping protein with regions of the cell containing free barbed ends as visualized by exogenous rhodamine-labeled G-actin is also observed after stimulation. An approximate threefold increase in the number of filaments with free barbed ends is accompanied by increases in absolute filament number, whereas the average filament length remains constant. Therefore, a mechanism in which preexisting filaments are uncapped by capping protein, in response to stimulation leading to the generation of free barbed ends and filament elongation, is not supported. A model for actin assembly after stimulation, whereby free barbed ends are generated by either filament severing or de novo nucleation is proposed. In this model, exposure of free barbed ends results in actin assembly, followed by entry of free capping protein into the actin cytoskeleton, which acts to terminate, not initiate, the actin polymerization transient.
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