ArticleThe Journal of cell biology1997
Molecular motors and a spectrin matrix associate with Golgi membranes in vitro.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 82 citations in OpenAlex.
- Small Sized Yet Powerful: Nuclear Distribution C Proteins in Plants.Plants (Basel, Switzerland) · 2023Review
- Fluorescence microscopy applied to intracellular transport by microtubule motors.Journal of biosciences · 2018Review
- Iterative sorting of apical and basolateral cargo in Madin-Darby canine kidney cells.Molecular biology of the cell · 2016Article
- Isoforms of Spectrin and Ankyrin Reflect the Functional Topography of the Mouse Kidney.PloS one · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Axonal transport and neurodegenerative disease: vesicle-motor complex formation and their regulation.Degenerative neurological and neuromuscular disease · 2014Review
- Protein partners of dynamin-1 in the retina.Visual neuroscience · 2013Article
- Cdc42 regulates microtubule-dependent Golgi positioning.Traffic (Copenhagen, Denmark) · 2010Article
- Targeted deletion of betaIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The mammalian NudC-like genes: a family with functions other than regulating nuclear distribution.Cellular and molecular life sciences : CMLS · 2009Review
- Ankyrin facilitates intracellular trafficking of alpha1-Na+-K+-ATPase in polarized cells.American journal of physiology. Cell physiology · 2008Article
- The unique architecture of Bunyamwera virus factories around the Golgi complex.Cellular microbiology · 2008Article
- Critical interactions between TGF-beta signaling/ELF, and E-cadherin/beta-catenin mediated tumor suppression.Oncogene · 2006Article
- Article
- Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct.Molecular & cellular proteomics : MCP · 2005Article
- Coatomer-bound Cdc42 regulates dynein recruitment to COPI vesicles.The Journal of cell biology · 2005Article
- Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts.The Journal of cell biology · 2002Article
- Article
- Actin dependence of polarized receptor recycling in Madin-Darby canine kidney cell endosomes.Molecular biology of the cell · 2002Article
- KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic dynein is a microtubule minus-end-directed motor that is thought to power the transport of vesicles from the TGN to the apical cortex in polarized epithelial cells. Trans-Golgi enriched membranes, which were isolated from primary polarized intestinal epithelial cells, contain both the actin-based motor myosin-I and dynein, whereas isolated Golgi stacks lack dynein but contain myosin-I (Fath, K.R., G.M. Trimbur, and D.R. Burgess. 1994. J. Cell Biol. 126:661-675). We show now that Golgi stacks in vitro bind dynein supplied from cytosol in the absence of ATP, and bud small membranes when incubated with cytosol and ATP. Cytosolic dynein binds to regions of stacks that are destined to bud because dynein is present in budded membranes, but absent from stacks after budding. Budded membranes move exclusively towards microtubule minus-ends in in vitro motility assays. Extraction studies suggest that dynein binds to a Golgi peripheral membrane protein(s) that resists extraction by ice-cold Triton X-100. In the presence of cytosol, these membrane ghosts can move towards the minus-ends of microtubules. Detergent-extracted Golgi stacks and TGN-containing membranes are closely associated with an amorphous matrix composed in part of spectrin and ankyrin. Although spectrin has been proposed to help link dynein to organellar membranes, we found that functional dynein may bind to extracted membranes independently of spectrin and ankyrin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.