ArticleThe Journal of cell biology1997
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 117 citations in OpenAlex.
- Mitotic dynamics of the nuclear lamina in the backstage of chromosome separation.Communications biology · 2025Review
- Crosstalk between mitotic reassembly and repair of the nuclear envelope.Nucleus (Austin, Tex.) · 2024Review
- Mechanobiology of the nucleus during the G2-M transition.Nucleus (Austin, Tex.) · 2024Review
- Nucleus size and its effect on nucleosome stability in living cells.Biophysical journal · 2022Article
- Cell Senescence and Central Regulators of Immune Response.International journal of molecular sciences · 2022Review
- The Diverse Cellular Functions of Inner Nuclear Membrane Proteins.Cold Spring Harbor perspectives in biology · 2021Review
- Increased expression of LAP2β eliminates nuclear membrane ruptures in nuclear lamin-deficient neurons and fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Host Cell S Phase RestrictsmBio · 2017Article
- Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability.Protoplasma · 2016Article
- Induction of polyploidy by nuclear fusion mechanism upon decreased expression of the nuclear envelope protein LAP2β in the human osteosarcoma cell line U2OS.Molecular cytogenetics · 2014Article
- Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope.Cell and tissue research · 2011Article
- Sizing up the nucleus: nuclear shape, size and nuclear-envelope assembly.Journal of cell science · 2009Review
- Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation.Biophysical journal · 2008Article
- The size-wise nucleus: nuclear volume control in eukaryotes.The Journal of cell biology · 2007Article
- TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts.The Journal of cell biology · 2006Article
- Dynamic nature of cleavage bodies and their spatial relationship to DDX1 bodies, Cajal bodies, and gems.Molecular biology of the cell · 2006Article
- Identification and functional evaluation of cellular and viral factors involved in the alteration of nuclear architecture during herpes simplex virus 1 infection.Journal of virology · 2005Article
- The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth.The EMBO journal · 2005Article
- Economy, speed and size matter: evolutionary forces driving nuclear genome miniaturization and expansion.Annals of botany · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Lamina-associated polypeptide 2 (LAP2) is an integral membrane protein of the inner nuclear membrane that binds to both lamin B and chromatin and has a putative role in nuclear envelope (NE) organization. We found that microinjection of a recombinant polypeptide comprising the nucleoplasmic domain of rat LAP2 (residues 1-398) into metaphase HeLa cells does not affect the reassembly of transport-competent nuclei containing NEs and lamina, but strongly inhibits nuclear volume increase. This effect appears to be specifically due to lamin binding, because it also is caused by microinjection of the minimal lamin-binding region of LAP2 (residues 298-373) but not by the chromatin-binding domain (residues 1-88). Injection of the lamin-binding region of rat LAP2 into early G1 phase HeLa cells also strongly affects nuclear growth; it almost completely prevents the threefold nuclear volume increase that normally occurs during the ensuing 10 h. Moreover, injection of the fragment during early G1 phase strongly inhibits entry of cells into S phase, whereas injection during S phase has no apparent effect on ongoing DNA replication. Since the lamin-binding fragment of LAP2 most likely acts by inhibiting dynamics of the nuclear lamina, our results suggest that a normal function of LAP2 involves regulation of nuclear lamina growth. These data also suggest that lamina dynamics are required for growth of the NE and for nuclear volume increase during the cell cycle, and that progression into S phase is dependent on the acquisition of a certain nuclear volume.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.