ArticleProceedings of the National Academy of Sciences of the United States of America1997
Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
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- Quantitative analysis of PPT1 interactome in human neuroblastoma cells.Data in brief · 2015Article
- Efficient in vitro gene delivery by hybrid biopolymer/virus nanobiovectors.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Design of hybrid lipid/retroviral-like particle gene delivery vectors.Molecular pharmaceutics · 2013Article
- Cell Membrane-associated heparan sulfate is a receptor for prototype foamy virus in human, monkey, and rodent cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Article
- Assembly of hepatitis B virus envelope proteins onto a lentivirus pseudotype that infects primary human hepatocytes.Journal of virology · 2007Article
- Enhanced transgenesis by intracytoplasmic injection of envelope-free lentivirus.Genesis (New York, N.Y. : 2000) · 2007Article
- Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein.Journal of virology · 2004Article
- Separable mechanisms of attachment and cell uptake during retrovirus infection.Journal of virology · 2000Article
- Association of murine leukemia virus pol with virions, independent of Gag-Pol expression.Journal of virology · 1999Article
- In vitro cell-free conversion of noninfectious Moloney retrovirus particles to an infectious form by the addition of the vesicular stomatitis virus surrogate envelope G protein.Journal of virology · 1998Article
- Enhanced gene transfer with fusogenic liposomes containing vesicular stomatitis virus G glycoprotein.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse transcriptase, and particles made by cells expressing an integrated lacZ provirus also contain viral RNA. The virus-like particles from both cell types are enveloped and are secreted/budded into the extracellular space but are noninfectious. Their physicochemical properties are similar to those of mature retroviral particles. The noninfectious gag pol RNA particles can readily be made infectious by the addition of lipofection reagents to produce preparations with titers of up to 10(5) colony-forming units per ml.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.