ArticleProceedings of the National Academy of Sciences of the United States of America1997
Analysis of exocytosis mutants indicates close coupling between regulated secretion and transcription activation in Tetrahymena.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Maturation stage-specific V-ATPase disassembly explains the neutral pH of mature mucocyst lysosome-related organelles in Tetrahymena thermophila.Journal of cell science · 2025Article
- An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma.The EMBO journal · 2022Article
- Lysosomal sorting receptors are essential for secretory granule biogenesis in Tetrahymena.The Journal of cell biology · 2013Article
- Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.Methods in cell biology · 2012Article
- GTP avoidance in Tetrahymena thermophila requires tyrosine kinase activity, intracellular calcium, NOS, and guanylyl cyclase.Purinergic signalling · 2008Article
- Core formation and the acquisition of fusion competence are linked during secretory granule maturation in Tetrahymena.Traffic (Copenhagen, Denmark) · 2005Article
- Transcriptional control of RAD51 expression in the ciliate Tetrahymena thermophila.Nucleic acids research · 2004Article
- Hypoxia regulates assembly of cilia in suppressors of Tetrahymena lacking an intraflagellar transport subunit gene.Molecular biology of the cell · 2003Article
- New class of cargo protein in Tetrahymena thermophila dense core secretory granules.Eukaryotic cell · 2002Article
- Exocyst is involved in cystogenesis and tubulogenesis and acts by modulating synthesis and delivery of basolateral plasma membrane and secretory proteins.Molecular biology of the cell · 2000Article
- Article
- Kinesin-II is preferentially targeted to assembling cilia and is required for ciliogenesis and normal cytokinesis in Tetrahymena.Molecular biology of the cell · 1999Article
- Identification and characterization of the RAD51 gene from the ciliate Tetrahymena thermophila.Nucleic acids research · 1998Article
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
- Proteolytic processing and Ca2+-binding activity of dense-core vesicle polypeptides in Tetrahymena.Molecular biology of the cell · 1998Article
- Tetrahymena: the key to the genetic analysis of the regulated pathway of polypeptide secretion?Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Stimulation of regulated secretory cells promotes protein release via the fusion of cytoplasmic storage vesicles with the plasma membrane. In Tetrahymena thermophila, brief exposure to secretagogue results in synchronous fusion of the entire set of docked dense-core granules with the plasma membrane. We show that stimulation is followed by rapid new dense-core granule synthesis involving gene induction. Two genes encoding granule matrix proteins, GRL1 and GRL4, are shown to undergo induction following stimulation, resulting in approximately 10-fold message accumulation within 1 h. The mechanism of induction involves transcriptional regulation, and the upstream region of GRL1 functions in vivo as an inducible promoter in a heterologous reporter construct using the gene encoding green fluorescent protein. Taking advantage of the characterized exocytosis (exo-) mutants available in this system, we asked whether the signals for regranulation were generated directly by the initial stimulation, or whether downstream events were required for transcription activation. Three mutants, with defects at three distinct stages in the regulated secretory pathway, failed to show induction of GRL1 and GRL4 after exposure to secretagogue. These results argue that regranulation depends upon signals generated by the final steps in exocytosis.
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