ArticleProceedings of the National Academy of Sciences of the United States of America1997
Fluorescence and photobleaching dynamics of single light-harvesting complexes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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Who cites it
45 citing papers in PubMed, 232 citations in OpenAlex.
- Deep Learning Parameter Estimation and Quantum Control of Single Molecules.ACS physical chemistry Au · 2026Article
- Observation of conformational dynamics in single light-harvesting proteins from cryptophyte algae.The Journal of chemical physics · 2022Article
- Hydrogel Fluorescence Microsensor with Fluorescence Recovery for Prolonged Stable Temperature Measurements.Sensors (Basel, Switzerland) · 2019Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- The contributions of 49ers to the measurements and models of ultrafast photosynthetic energy transfer.Photosynthesis research · 2018Article
- How reduced excitonic coupling enhances light harvesting in the main photosynthetic antennae of diatoms.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Quenching Capabilities of Long-Chain Carotenoids in Light-Harvesting-2 Complexes from Rhodobacter sphaeroides with an Engineered Carotenoid Synthesis Pathway.The journal of physical chemistry. B · 2016Article
- Pushing the Photon Limit: Nanoantennas Increase Maximal Photon Stream and Total Photon Number.The journal of physical chemistry letters · 2016Article
- Up-converted fluorescence from photosynthetic light-harvesting complexes linearly dependent on excitation intensity.Photosynthesis research · 2016Article
- Dissecting pigment architecture of individual photosynthetic antenna complexes in solution.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Principles of light harvesting from single photosynthetic complexes.Interface focus · 2015Review
- Conformational switching in a light-harvesting protein as followed by single-molecule spectroscopy.Biophysical journal · 2015Article
- Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.Biochemistry · 2013Review
- The specificity of controlled protein disorder in the photoprotection of plants.Biophysical journal · 2013Article
- Single-molecule spectroscopy reveals photosynthetic LH2 complexes switch between emissive states.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Polarization single complex imaging of circular photosynthetic antenna.Photosynthesis research · 2012Article
- Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Ultrafast time-resolved spectroscopy of the light-harvesting complex 2 (LH2) from the photosynthetic bacterium Thermochromatium tepidum.Photosynthesis research · 2011Article
- Watching conformational- and photo-dynamics of single fluorescent proteins in solution.Nature chemistry · 2010Article
- Revealing linear aggregates of light harvesting antenna proteins in photosynthetic membranes.Langmuir : the ACS journal of surfaces and colloids · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Single light-harvesting complexes LH-2 from Rhodopseudomonas acidophila were immobilized on various charged surfaces under physiological conditions. Polarized light experiments showed that the complexes were situated on the surface as nearly upright cylinders. Their fluorescence lifetimes and photobleaching properties were obtained by using a confocal fluorescence microscope with picosecond time resolution. Initially all molecules fluoresced with a lifetime of 1 +/- 0.2 ns, similar to the bulk value. The photobleaching of one bacteriochlorophyll molecule from the 18-member assembly caused the fluorescence to switch off completely, because of trapping of the mobile excitations by energy transfer. This process was linear in light intensity. On continued irradiation the fluorescence often reappeared, but all molecules did not show the same behavior. Some LH-2 complexes displayed a variation of their quantum yields that was attributed to photoinduced confinement of the excited states and thereby a diminution of the superradiance. Others showed much shorter lifetimes caused by excitation energy traps that are only approximately 3% efficient. On repeated excitation some molecules entered a noisy state where the fluorescence switched on and off with a correlation time of approximately 0.1 s. About 490 molecules were examined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.