ArticleProceedings of the National Academy of Sciences of the United States of America1997
Both DNA gyrase and reverse gyrase are present in the hyperthermophilic bacterium Thermotoga maritima.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- How Do Thermophiles Organize Their Genomes?Microbes and environments · 2024Review
- Genomic attributes of thermophilic and hyperthermophilic bacteria and archaea.World journal of microbiology & biotechnology · 2022Review
- The hyperthermophilic archaeon Thermococcus kodakarensis is resistant to pervasive negative supercoiling activity of DNA gyrase.Nucleic acids research · 2021Article
- The regulation of DNA supercoiling across evolution.Protein science : a publication of the Protein Society · 2021Review
- Single-molecule insights into torsion and roadblocks in bacterial transcript elongation.Transcription · 2021Article
- The Evolution of Reverse Gyrase Suggests a Nonhyperthermophilic Last Universal Common Ancestor.Molecular biology and evolution · 2019Article
- Reverse gyrase is essential for microbial growth at 95 °C.Extremophiles : life under extreme conditions · 2017Article
- Torsion-mediated interaction between adjacent genes.PLoS computational biology · 2014Article
- Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.Extremophiles : life under extreme conditions · 2014Review
- Reverse gyrase--recent advances and current mechanistic understanding of positive DNA supercoiling.Nucleic acids research · 2014Review
- DNA self-assembly: from chirality to evolution.International journal of molecular sciences · 2013Review
- Article
- The linkage between reverse gyrase and hyperthermophiles: a review of their invariable association.Journal of microbiology (Seoul, Korea) · 2009Review
- Phylogenomics of DNA topoisomerases: their origin and putative roles in the emergence of modern organisms.Nucleic acids research · 2009Review
- DNA protection by histone-like protein HU from the hyperthermophilic eubacterium Thermotoga maritima.Nucleic acids research · 2008Article
- Widespread distribution of archaeal reverse gyrase in thermophilic bacteria suggests a complex history of vertical inheritance and lateral gene transfers.Archaea (Vancouver, B.C.) · 2007Article
- DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus.Nucleic acids research · 2002Article
- Plasmid pGS5 from the hyperthermophilic archaeon Archaeoglobus profundus is negatively supercoiled.Journal of bacteriology · 2000Article
- Structural analysis of DNA sequence: evidence for lateral gene transfer in Thermotoga maritima.Nucleic acids research · 2000Article
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5 authors.
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Abstract
Like all hyperthermophiles yet tested, the bacterium Thermotoga maritima contains a reverse gyrase. Here we show that it contains also a DNA gyrase. The genes top2A and top2B encoding the two subunits of a DNA gyrase-like enzyme have been cloned and sequenced. The Top2A (type II DNA topoisomerase A protein) is more similar to GyrA (DNA gyrase A protein) than to ParC [topoisomerase IV (Topo IV) C protein]. The difference is especially striking at the C-terminal domain, which differentiates DNA gyrases from Topo IV. DNA gyrase activity was detected in T. maritima and purified to homogeneity using a novobiocin-Sepharose column. This hyperhermophilic DNA gyrase has an optimal activity around 82-86 degrees C. In contrast to plasmids from hyperthermophilic archaea, which are from relaxed to positively supercoiled, we found that the plasmid pRQ7 from Thermotoga sp. RQ7 is negatively supercoiled. pRQ7 became positively supercoiled after addition of novobiocin to cell cultures, indicating that its negative supercoiling is due to the DNA gyrase of the host strain. The findings concerning DNA gyrase and negative supercoiling in Thermotogales put into question the role of reverse gyrase in hyperthermophiles.
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