ArticleNucleic acids research1997
DNA binding and transcriptional activation by the Ski oncoprotein mediated by interaction with NFI.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Nat10-mediated ac4C epitranscriptomics orchestrates hematopoietic stem/progenitor cell fate determination via translation control.Science advances · 2026Article
- PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB.FEBS letters · 2024Article
- Aortic root dilatation and dilated cardiomyopathy in an adult with Tatton-Brown-Rahman syndrome.American journal of medical genetics. Part A · 2022Article
- Ski mediates TGF-β1-induced fibrosarcoma cell proliferation and promotes tumor growth.Journal of Cancer · 2020Article
- Further delineation of Malan syndrome.Human mutation · 2018Article
- MYB induces the expression of the oncogenic corepressor SKI in acute myeloid leukemia.Oncotarget · 2018Article
- Combined cistrome and transcriptome analysis of SKI in AML cells identifies SKI as a co-repressor for RUNX1.Nucleic acids research · 2018Article
- Article
- Ski protein levels increase during in vitro progression of HPV16-immortalized human keratinocytes and in cervical cancer.Virology · 2013Article
- SnoN signaling in proliferating cells and postmitotic neurons.FEBS letters · 2012Review
- CTF/NF1 transcription factors act as potent genetic insulators for integrating gene transfer vectors.Gene therapy · 2012Article
- Nuclear factor I-A represses expression of the cell adhesion molecule L1.BMC molecular biology · 2009Article
- Nuclear factor I-C links platelet-derived growth factor and transforming growth factor beta1 signaling to skin wound healing progression.Molecular and cellular biology · 2009Article
- DACH1 is a cell fate determination factor that inhibits cyclin D1 and breast tumor growth.Molecular and cellular biology · 2006Article
- NFI-Ski interactions mediate transforming growth factor beta modulation of human papillomavirus type 16 early gene expression.Journal of virology · 2004Article
- Defective T-cell activation is associated with augmented transforming growth factor Beta sensitivity in mice with mutations in the Sno gene.Molecular and cellular biology · 2003Article
- Ski interacts with the evolutionarily conserved SNW domain of Skip.Nucleic acids research · 2001Article
- The Ski oncoprotein interacts with the Smad proteins to repress TGFbeta signaling.Genes & development · 1999Article
- Transformation of hematopoietic cells by the Ski oncoprotein involves repression of retinoic acid receptor signaling.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Transcriptional cofactors Ski and SnoN are major regulators of the TGF-β/Smad signaling pathway in health and disease.Signal transduction and targeted therapyArticle
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
Abstract
The Ski oncoprotein has been found to bind non-specifically to DNA in association with unindentified nuclear factors. In addition, Ski has been shown to activate transcription of muscle-specific and viral promoters/enhancers. The present study was undertaken to identify Ski's DNA binding and transcriptional activation partners by identifying specific DNA binding sites. We used nuclear extracts from a v-Ski-transduced mouse L-cell line and selected Ski-bound sequences from a pool of degenerate oligonucleotides with anti-Ski monoclonal antibodies. Two sequences were identified by this technique. The first (TGGC/ANNNNNT/GCCAA) is the previously identified binding site of the nuclear factor I (NFI) family of transcription factors. The second (TCCCNNGGGA) is the binding site of Olf-1/EBF. By electophoretic mobility shift assays we find that Ski is a component of one or more NFI complexes but we fail to detect Ski in Olf-1/EBF complexes. We show that Ski binds NFI proteins and activates transcription of NFI reporters, but only in the presence of NFI. We also find that homodimerization of Ski is essential for co-activation with NFI. However, the C-terminal dimerization domain of c-Ski, which is missing in v-Ski, can be substituted by the leucine zipper domain of GCN4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.