ArticleThe Journal of physiology1997
Swelling- and cAMP-activated Cl- currents in isolated rat carotid body type I cells.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Cell swelling enhances ligand-driven β-adrenergic signaling.Nature communications · 2024Article
- Mechanisms of Chemosensory Transduction in the Carotid Body.Advances in anatomy, embryology, and cell biology · 2023Article
- A 30-year journey from volume-regulated anion currents to molecular structure of the LRRC8 channel.The Journal of general physiology · 2019Review
- Carotid Body Type-I Cells Under Chronic Sustained Hypoxia: Focus on Metabolism and Membrane Excitability.Frontiers in physiology · 2018Article
- ATP release during cell swelling activates a CaScientific reports · 2017Article
- TASK channels in arterial chemoreceptors and their role in oxygen and acid sensing.Pflugers Archiv : European journal of physiology · 2015Review
- Revisiting cAMP signaling in the carotid body.Frontiers in physiology · 2014Review
- Peripheral chemoreceptors: function and plasticity of the carotid body.Comprehensive Physiology · 2012Review
- AQP1 mediates water transport in the carotid body.Pflugers Archiv : European journal of physiology · 2010Article
- EPAC signalling pathways are involved in low PO2 chemoreception in carotid body chemoreceptor cells.The Journal of physiology · 2009Article
- Volume-activated chloride channels in mice Leydig cells.Pflugers Archiv : European journal of physiology · 2008Article
- Volume-sensitive Cl- current in bovine adrenocortical cells: comparison with the ACTH-induced Cl- current.The Journal of membrane biology · 2004Article
- Effects of osmotic changes on the chemoreceptor cell of rat carotid body.The Journal of physiology · 2003Article
- A pH-sensitive chloride current in the chemoreceptor cell of rat carotid body.The Journal of physiology · 2001Article
- Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD).The Journal of physiology · 2001Review
- Activation of volume-regulated Cl(-) channels by ACh and ATP in Xenopus follicles.The Journal of physiology · 2000Article
- Effects of hypoxia and dithionite on catecholamine release from isolated type I cells of the rat carotid body.The Journal of physiology · 2000Article
- ATP dependence of the ICl,swell channel varies with rate of cell swelling. Evidence for two modes of channel activation.The Journal of general physiology · 1999Article
- Synergetic activation of outwardly rectifying Cl- currents by hypotonic stress and external Ca2+ in murine osteoclasts.The Journal of physiology · 1999Article
- Inhibition of Ca2+-dependent K+ channels in rat carotid body type I cells by protein kinase C.The Journal of physiology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. In the whole-cell configuration of the patch clamp technique, isolated rat carotid body type I cells exhibited reversible activation of Cl- currents during cell swelling effected by hypotonic extracellular solutions. 2. Hypotonic solutions evoked outwardly rectifying, non-inactivating currents which showed time-independent activation. The reversal potential (E(rev)) for the hypotonically evoked current was 1.6 +/- 0.6 mV (n = 26). Reduction of extracellular Cl- from 133 to 65.5 mM caused a shift in E(rev) of +14.7 +/- 0.4 mV (n = 5). 3. The swelling-activated Cl- current could not activate when ATP was omitted from the patch pipette or when substituted for the non-hydrolysable ATP analogues 5'-adenylylimidodiphosphate, AMP-PNP (2 mM) or beta, gamma-methylene-adenosine 5'-triphosphate. AMP-PCP (2 mM). The current also failed to activate in the absence of free intracellular Ca2+. 4. The swelling-activated Cl- current was sensitive to blockade by the Cl- channel blockers niflumic acid (300 microM) and 4,4'-diisothiocyanatostilbene-2, 2'-disulphonic acid (DIDS; 200 microM), although the blockade by DIDS was voltage dependent. 5. A similar, non-inactivating, outwardly rectifying Cl- current was evoked by the inclusion of cAMP (200 microM) in the patch pipette. This current could be inhibited by niflumic acid (300 microM), DIDS (200 microM) and hypertonic solutions, and was virtually abolished in the absence of intracellular ATP. 6. In conclusion, carotid body type I cells possess Cl- currents activated by cell swelling and rises in intracellular cAMP concentration. These currents may be involved in cell volume regulation, blood volume and osmolarity regulation and the response of the type I cell to chemostimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.