ArticleMolecular and cellular biology1997
The yeast silent information regulator Sir4p anchors and partitions plasmids.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- The yeast 2-micron plasmid Rep2 protein has Rep1-independent partitioning function.Nucleic acids research · 2022Article
- Replicative aging is associated with loss of genetic heterogeneity from extrachromosomal circular DNA in Saccharomyces cerevisiae.Nucleic acids research · 2020Article
- The Sir4 H-BRCT domain interacts with phospho-proteins to sequester and repress yeast heterochromatin.The EMBO journal · 2019Article
- Discovery and Evolution of New Domains in Yeast Heterochromatin Factor Sir4 and Its Partner Esc1.Genome biology and evolution · 2019Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- High Throughput Analyses of Budding Yeast ARSs Reveal New DNA Elements Capable of Conferring Centromere-Independent Plasmid Propagation.G3 (Bethesda, Md.) · 2016Article
- Asymmetrical Inheritance of Plasmids Depends on Dynamic Cellular Geometry and Volume Exclusion Effects.PloS one · 2015Article
- Epigenetics in Saccharomyces cerevisiae.Cold Spring Harbor perspectives in biology · 2013Review
- Review
- Reinventing heterochromatin in budding yeasts: Sir2 and the origin recognition complex take center stage.Eukaryotic cell · 2011Review
- The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.Nature cell biology · 2011Article
- Double rolling circle replication (DRCR) is recombinogenic.Genes to cells : devoted to molecular & cellular mechanisms · 2011Article
- Early initiation of a replication origin tethered at the nuclear periphery.Journal of cell science · 2010Article
- Retrotransposon target site selection by imitation of a cellular protein.Molecular and cellular biology · 2008Article
- A tDNA establishes cohesion of a neighboring silent chromatin domain.Genes & development · 2007Article
- SIR2 modifies histone H4-K16 acetylation and affects superhelicity in the ARS region of plasmid chromatin in Saccharomyces cerevisiae.Nucleic acids research · 2006Article
- The amino terminus of Epstein-Barr Virus (EBV) nuclear antigen 1 contains AT hooks that facilitate the replication and partitioning of latent EBV genomes by tethering them to cellular chromosomes.Journal of virology · 2004Article
- Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins.The EMBO journal · 2004Article
- REP3-mediated silencing in Saccharomyces cerevisiae.Genetics · 2004Article
- CEN plasmid segregation is destabilized by tethered determinants of Ty 5 integration specificity: a role for double-strand breaks in CEN antagonism.Chromosoma · 2003Article
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2 authors.
Funding
Abstract
Circular plasmids containing telomeric TG1-3 arrays or the HMR E silencer segregate efficiently between dividing cells of the yeast Saccharomyces cerevisiae. Subtelomeric X repeats augment the TG1-3 partitioning activity by a process that requires the SIR2, SIR3, and SIR4 genes, which are also required for silencer-based partitioning. Here we show that targeting Sir4p to DNA directly via fusion to the bacterial repressor LexA confers efficient mitotic segregation to otherwise unstable plasmids. The Sir4p partitioning activity resides within a 300-amino-acid region (residues 950 to 1262) which precedes the coiled-coil dimerization motif at the extreme carboxy end of the protein. Using a topology-based assay, we demonstrate that the partitioning domain also retards the axial rotation of LexA operators in vivo. The anchoring and partitioning properties of LexA-Sir4p chimeras persist despite the loss of the endogenous SIR genes, indicating that these functions are intrinsic to Sir4p and not to a complex of Sir factors. In contrast, inactivation of the Sir4p-interacting protein Rap1p reduces partitioning by a LexA-Sir4p fusion. The data are consistent with a model in which the partitioning and anchoring domain of Sir4p (PAD4 domain) attaches to a nuclear component that divides symmetrically between cells at mitosis; DNA linked to Sir4p by LexA serves as a reporter of protein movement in these experiments. We infer that the segregation behavior of telomere- and silencer-based plasmids is, in part, a consequence of these Sir4p-mediated interactions. The assays presented herein illustrate two novel approaches to monitor the intracellular dynamics of nuclear proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.