ArticleMolecular and cellular biology1997
Promoter activity of Tat at steps subsequent to TATA-binding protein recruitment.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- HIV-1 Proviral Transcription and Latency in the New Era.Viruses · 2020Review
- Cat and Mouse: HIV Transcription in Latency, Immune Evasion and Cure/Remission Strategies.Viruses · 2019Review
- Transition step during assembly of HIV Tat:P-TEFb transcription complexes and transfer to TAR RNA.Molecular and cellular biology · 2012Article
- CTGC motifs within the HIV core promoter specify Tat-responsive pre-initiation complexes.Retrovirology · 2012Article
- The HIV-1 transcriptional activator Tat has potent nucleic acid chaperoning activities in vitro.Nucleic acids research · 2008Article
- Tat gets the "green" light on transcription initiation.Retrovirology · 2005Review
- Regulation of human immunodeficiency virus type 1 gene expression by clade-specific Tat proteins.Journal of virology · 2005Article
- Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex.Retrovirology · 2004Article
- Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.The EMBO journal · 2002Article
- Human immunodeficiency virus type 1 nucleocapsid protein nuclear localization mediates early viral mRNA expression.Journal of virology · 2002Article
- Rice TATA binding protein interacts functionally with transcription factor IIB and the RF2a bZIP transcriptional activator in an enhanced plant in vitro transcription system.The Plant cell · 2002Article
- Artificially recruited TATA-binding protein fails to remodel chromatin and does not activate three promoters that require chromatin remodeling.Molecular and cellular biology · 2000Article
- Hepatitis C virus core protein-induced loss of LZIP function correlates with cellular transformation.The EMBO journal · 2000Article
- Role of the TATA binding protein-transcription factor IIB interaction in supporting basal and activated transcription in plant cells.The Plant cell · 2000Article
- The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties.Oncogene · 1999Article
- Transcriptional regulation by targeted recruitment of cyclin-dependent CDK9 kinase in vivo.Oncogene · 1999Article
- Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure.Molecular and cellular biology · 1999Article
- Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a TATA-box-dependent manner.Molecular and cellular biology · 1999Article
- Cell cycle- and Vpr-mediated regulation of human immunodeficiency virus type 1 expression in primary and transformed T-cell lines.Journal of virology · 1999Article
- Transcriptional activation by artificial recruitment in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artificial recruitment of TATA-binding protein (TBP) to many eukaryotic promoters bypasses DNA-bound activator function. The human immunodeficiency virus type 1 (HIV-1) Tat is an unconventional activator that up-regulates transcription from the HIV-1 long terminal repeat (LTR) through binding to a nascent RNA sequence, TAR. Because this LTR and its cognate activator have atypical features compared to a standard RNA polymerase II (RNAP II) transcriptional unit, the precise limiting steps for HIV-1 transcription and how Tat resolves these limitations remain incompletely understood. We thus constructed human TBP fused to the DNA-binding domain of GAL4 to determine whether recruitment of TBP is one rate-limiting step in HIV-1 LTR transcription and whether Tat functions to recruit TBP. As a control, we compared the activity of the adenovirus E1b promoter. Our findings indicate that TBP tethering to the E1b promoter fully effected transcription to the same degree achievable with the potent GAL4-VP16 activator. By contrast, TBP recruitment to the HIV-1 LTR, although necessary for conferring Tat responsiveness, did not bypass a physical need for Tat in achieving activated transcription. These results document that the HIV-1 and the E1b promoters are transcriptionally limited at different steps; the major rate-limiting step for E1b is recruitment of TBP, while activation of the HIV-1 LTR requires steps in addition to TBP recruitment. We suggest that Tat acts to accelerate rate-limiting steps after TBP recruitment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.