ArticleProceedings of the National Academy of Sciences of the United States of America1997
A deletion in an indole synthase gene is responsible for the DIMBOA-deficient phenotype of bxbx maize.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 53 citations in OpenAlex.
- A pseudoenzyme enables indole biosynthesis in eudicot plants.Nature chemical biology · 2026Article
- Microbes Associated to Dyer's woad (Isatis tinctoria L.): Pigment Extraction, Dyeing and Cultivation with Non-toxic Inputs. A Review.Current microbiology · 2025Review
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Synthesis and regulation of auxin and abscisic acid in maize.Plant signaling & behavior · 2021Review
- Contrasting insect attraction and herbivore-induced plant volatile production in maize.Planta · 2018Article
- A Feedback-Insensitive Isopropylmalate Synthase Affects Acylsugar Composition in Cultivated and Wild Tomato.Plant physiology · 2015Article
- Integrative Approaches to Enhance Understanding of Plant Metabolic Pathway Structure and Regulation.Plant physiology · 2015Article
- Something Old, Something New: Conserved Enzymes and the Evolution of Novelty in Plant Specialized Metabolism.Plant physiology · 2015Review
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
- Dispersed benzoxazinone gene cluster: molecular characterization and chromosomal localization of glucosyltransferase and glucosidase genes in wheat and rye.Plant physiology · 2011Article
- Benzoxazinoid metabolites regulate innate immunity against aphids and fungi in maize.Plant physiology · 2011Article
- Novel acidic sesquiterpenoids constitute a dominant class of pathogen-induced phytoalexins in maize.Plant physiology · 2011Article
- ZmPep1, an ortholog of Arabidopsis elicitor peptide 1, regulates maize innate immunity and enhances disease resistance.Plant physiology · 2011Article
- Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: characterization of Bx6 and Bx7.Plant physiology · 2008Article
- Structures of the three homoeologous loci of wheat benzoxazinone biosynthetic genes TaBx3 and TaBx4 and characterization of their promoter sequences.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Three genomes differentially contribute to the biosynthesis of benzoxazinones in hexaploid wheat.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Hydroxamic acid content and toxicity of rye at selected growth stages.Journal of chemical ecology · 2005Article
- Auxin: regulation, action, and interaction.Annals of botany · 2005Review
- Changes over time in the allelochemical content of ten cultivars of rye (Secale cereale L.).Journal of chemical ecology · 2005Article
- In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the alpha subunits OASA1 and OASA2.Plant molecular biology · 2004Article
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4 authors at 1 institution in 1 country.
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Abstract
The biosynthesis of DIMBOA, a pesticidal secondary metabolite of maize, branches off the tryptophan pathway. We have previously demonstrated that indole is the last intermediate common to both the tryptophan and hydroxamic acid pathways. The earliest discovered mutant in the DIMBOA pathway, bxbx (benzoxazineless), is deficient in the production of DIMBOA and related compounds. This paper presents evidence that a gene identified by Kramer and Koziel [Kramer, V. C. & Koziel, M. G. (1995) Plant Mol. Biol. 27, 1183-1188] as maize tryptophan synthase alpha (TSA) is the site of the genetic lesion in the DIMBOA-deficient mutant maize line bxbx. We demonstrate that the TSA gene has sustained a 924-bp deletion in bxbx compared with its counterpart in wild-type maize. We report that the TSA gene maps to the same location as the bxbx mutation, on the short arm of chromosome 4. We present evidence that the very early and very high level of expression of TSA corresponds to the timing and level of DIMBOA biosynthesis but is strikingly different from the expression of the maize tryptophan synthase beta (TSB) genes. We show that feeding indole to bxbx seedlings restores their ability to synthesize DIMBOA. We conclude that the maize enzyme initially named tryptophan synthase alpha in fact is a DIMBOA biosynthetic enzyme, and we propose that it be renamed indole synthase. This work confirms and enlarges upon the findings of Frey et al. [Frey, M., Chomet, P., Glawischniq, E., Stettner, C., Grün, S., Winklmair, A., Eisenreich, W., Bacher, A., Meeley, R. B., Briggs, S. P., Simcox, K. & Gierl, A. (1997) Science 277, 696-699], which appeared while the present paper was in review.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.