ArticleProceedings of the National Academy of Sciences of the United States of America1997
Gi regulation of secretory vesicle swelling examined by atomic force microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 116 citations in OpenAlex.
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- Human Platelet Vesicles Exhibit Distinct Size and Proteome.Journal of proteome research · 2017Article
- 'Porosome' discovered nearly 20 years ago provides molecular insights into the kiss-and-run mechanism of cell secretion.Journal of cellular and molecular medicine · 2015Review
- Discoveries Interview: Professor Bhanu P. Jena on the discovery of the porosome, the universal machinery for cellular secretion.Discoveries (Craiova, Romania) · 2014Article
- NeuronalJournal of molecular structure · 2014Article
- Proteome of the porosome complex in human airway epithelia: interaction with the cystic fibrosis transmembrane conductance regulator (CFTR).Journal of proteomics · 2014Article
- 3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.Histochemistry and cell biology · 2012Article
- Water channels in platelet volume regulation.Journal of cellular and molecular medicine · 2012Article
- Quantitative nanostructural and single-molecule force spectroscopy biomolecular analysis of human-saliva-derived exosomes.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Involvement of β-adrenergic receptor in synaptic vesicle swelling and implication in neurotransmitter release.Journal of cellular and molecular medicine · 2011Article
- Atomic force microscopy: Unraveling the fundamental principles governing secretion and membrane fusion in cells.Ultramicroscopy · 2009Review
- Single Molecule Probing of Exocytotic Protein Interactions Using Force Spectroscopy.Croatica chemica acta. Arhiv za kemiju · 2008Article
- Secretion machinery at the cell plasma membrane.Current opinion in structural biology · 2007Review
- AQPs and control of vesicle volume in secretory cells.The Journal of membrane biology · 2006Article
- Size of supramolecular SNARE complex: membrane-directed self-assembly.Journal of the American Chemical Society · 2005Article
- Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle.The Journal of physiology · 2000Article
- VMAT-Mediated changes in quantal size and vesicular volume.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last decade, several monomeric and heterotrimeric guanine nucleotide binding proteins have been identified to associate with secretory vesicles and to be implicated in exocytosis. Vesicle volume also has been proposed to play a regulatory role in secretory vesicle fusion at the plasma membrane. However, the molecular mechanism of function of the guanine nucleotide binding proteins and of the regulation of secretory vesicle volume in the exocytotic process remains unclear. In this study, we report association of the secretory vesicle membrane with the alpha subunit of a heterotrimeric GTP binding protein G(alpha i3) and implicate its involvement in vesicle swelling. Using an atomic force microscope in combination with confocal microscopy, we were able to study the dynamics of isolated zymogen granules, the secretory vesicles in exocrine pancreas. Exposure of zymogen granules to GTP resulted in a 15-25% increase in vesicle height as measured by the atomic force microscope and a similar increase in vesicle diameter as determined by confocal microscopy. Mas7, an active mastoparan analog known to stimulate Gi proteins, was found to stimulate the GTPase activity of isolated zymogen granules and cause swelling. Increase in vesicle size in the presence of GTP, NaF, and Mas7 were irreversible and KCl-sensitive. Ca2+ had no effect on zymogen granule size. Taken together, the results indicate that G(alpha i3) protein localized in the secretory vesicle membrane mediates vesicle swelling, a potentially important prerequisite for vesicle fusion at the cell plasma membrane.
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