ArticleProceedings of the National Academy of Sciences of the United States of America1997
TnTIN and TnTAP: mini-transposons for site-specific proteolysis in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- A Mini-ISY100 Transposon Delivery System Effective in γ Proteobacteria.Frontiers in microbiology · 2019Article
- Outer membrane lipoprotein biogenesis: Lol is not the end.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- The ABC transporter MalFGK(2) sequesters the MalT transcription factor at the membrane in the absence of cognate substrate.Molecular microbiology · 2012Article
- In situ cleavage of the acidic domain from the p115 tether inhibits exocytic transport.Traffic (Copenhagen, Denmark) · 2008Article
- The U1 snRNP-associated factor Luc7p affects 5' splice site selection in yeast and human.Nucleic acids research · 2007Article
- Towards the identification of type II secretion signals in a nonacylated variant of pullulanase from Klebsiella oxytoca.Journal of bacteriology · 2005Article
- Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast.BMC molecular biology · 2005Article
- Target-directed proteolysis at the ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Agrobacterium tumefaciens VirB6 domains direct the ordered export of a DNA substrate through a type IV secretion System.Journal of molecular biology · 2004Article
- The usher N terminus is the initial targeting site for chaperone-subunit complexes and participates in subsequent pilus biogenesis events.Journal of bacteriology · 2004Article
- Genome-wide internal tagging of bacterial exported proteins.Nature biotechnology · 2002Article
- Membrane topology of the p1258 CadA Cd(II)/Pb(II)/Zn(II)-translocating P-type ATPase.Journal of bioenergetics and biomembranes · 2002Article
- Evidence for coupling of membrane targeting and function of the signal recognition particle (SRP) receptor FtsY.EMBO reports · 2001Article
- Genetic dissection of the outer membrane secretin PulD: are there distinct domains for multimerization and secretion specificity?Journal of bacteriology · 1999Article
- Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.Journal of bacteriology · 1999Article
- Dislocation of membrane proteins in FtsH-mediated proteolysis.The EMBO journal · 1999Article
- Prediction by a neural network of outer membrane beta-strand protein topology.Protein science : a publication of the Protein Society · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Tobacco etch virus (TEV) protease recognizes a 7-aa consensus sequence, Glu-Xaa-Xaa-Tyr-Xaa-Gln-Ser, where Xaa can be almost any amino acyl residue. Cleavage occurs between the conserved Gln and Ser residues. Because of its distinct specificity, TEV protease can be expressed in the cytoplasm without interfering with viability. Polypeptides that are not natural substrates of TEV protease are proteolyzed if they carry the appropriate cleavage site. Thus, this protease can be used to study target proteins in their natural environment in vivo, as well as in vitro. We describe two TnS-based mini-transposons that insert TEV protease cleavage sites at random into target proteins. TnTIN introduces TEV cleavage sites into cytoplasmic proteins. TnTAP facilitates the same operation for proteins localized to the bacterial cell envelope. By using two different target proteins, SecA and TolC, we show that such modified proteins can be cleaved in vivo and in vitro by TEV protease. Possible applications of the site-specific proteolysis approach are topological studies of soluble as well as of inner and outer membrane proteins, protein inactivation, insertion mutagenesis experiments, and protein tagging.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.