ArticleProceedings of the National Academy of Sciences of the United States of America1997
Erythrocyte membrane vesiculation: model for the molecular mechanism of protein sorting.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 80 citations in OpenAlex.
- Proteomes of Micro- and Nanosized Carriers Engineered from Red Blood Cells.Journal of proteome research · 2023Article
- Reticulocyte Maturation.Membranes · 2022Review
- Red Blood Cells: Tethering, Vesiculation, and Disease in Micro-Vascular Flow.Diagnostics (Basel, Switzerland) · 2021Article
- Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles.Experimental mechanics · 2019Article
- ATP effects on response of human erythrocyte membrane to high pressure.Biophysics and physicobiology · 2019Article
- Computational modeling of single-cell mechanics and cytoskeletal mechanobiology.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Whole Blood Storage in CPDA1 Blood Bags Alters Erythrocyte Membrane Proteome.Oxidative medicine and cellular longevity · 2018Article
- Erythrocyte Aging, Protection via Vesiculation: An Analysis Methodology via Oscillatory Flow.Frontiers in physiology · 2018Article
- Antimicrobial peptides are degraded by the cytosolic proteases of human erythrocytes.Biochimica et biophysica acta. Biomembranes · 2017Article
- Prospects for Human Erythrocyte Skeleton-Bilayer Dissociation during Splenic Flow.Biophysical journal · 2017Article
- Acetylcholinesterase provides new insights into red blood cell ageing in vivo and in vitro.Blood transfusion = Trasfusione del sangue · 2017Article
- Dynamics of red blood cells in microporous membranes.Biomicrofluidics · 2014Article
- Red blood cell vesiculation in hereditary hemolytic anemia.Frontiers in physiology · 2013Review
- Lipid bilayer and cytoskeletal interactions in a red blood cell.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mechanics of membrane bulging during cell-wall disruption in gram-negative bacteria.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Multiscale simulation of erythrocyte membranes.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Autonomous functions for the Sec14p/spectrin-repeat region of Kalirin.Experimental cell research · 2008Article
- Tether extrusion from red blood cells: integral proteins unbinding from cytoskeleton.Biophysical journal · 2007Article
- Cytoskeletal dynamics of human erythrocyte.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Hydrodynamic narrowing of tubes extruded from cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Budding and vesiculation of erythrocyte membranes occurs by a process involving an uncoupling of the membrane skeleton from the lipid bilayer. Vesicle formation provides an important means whereby protein sorting and trafficking can occur. To understand the mechanism of sorting at the molecular level, we have developed a micropipette technique to quantify the redistribution of fluorescently labeled erythrocyte membrane components during mechanically induced membrane deformation and vesiculation. Our previous studies indicated that the spectrin-based membrane skeleton deforms elastically, producing a constant density gradient during deformation. Our current studies showed that during vesiculation the skeleton did not fragment but rather retracted to the cell body, resulting in a vesicle completely depleted of skeleton. These local changes in skeletal density regulated the sorting of nonskeletal membrane components. Highly mobile membrane components, phosphatidylethanolamine- and glycosylphosphatidylinositol-linked CD59 with no specific skeletal association were enriched in the vesicle. In contrast, two components with known specific skeletal association, band 3 and glycophorin A, were differentially depleted in vesicles. Increasing the skeletal association of glycophorin A by liganding its extrafacial domain reduced the fraction partitioning to the vesicle. We conclude that this technique of bilayer/skeleton uncoupling provides a means with which to study protein sorting driven by changes in local skeletal density. Moreover, it is the interaction of particular membrane components with the spectrin-based skeleton that determines molecular partitioning during protein sorting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.