ArticleGenome research1997
A simple method for automated allele binning in microsatellite markers.
Article in Genome research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- STRyper: A macOS application for microsatellite genotyping and chromatogram management.PloS one · 2025Article
- Population Genetic Analysis and Sub-Structuring ofFrontiers in genetics · 2021Article
- Analysis of in situ diversity and population structure in Ethiopian cultivated Sorghum bicolor (L.) landraces using phenotypic traits and SSR markers.SpringerPlus · 2014Article
- Range dynamics, rather than convergent selection, explain the mosaic distribution of red-winged blackbird phenotypes.Ecology and evolution · 2013Article
- Sangiovese and its offspring in southern Italy.Molecular biotechnology · 2013Article
- Estimation of in situ mating systems in wild sorghum (Sorghum bicolor (L.) Moench) in Ethiopia using SSR-based progeny array data: implications for the spread of crop genes into the wild.Journal of genetics · 2013Article
- Development and validation of microsatellite markers for Brachiaria ruziziensis obtained by partial genome assembly of Illumina single-end reads.BMC genomics · 2013Article
- A study of the relationships of cultivated peanut (Arachis hypogaea) and its most closely related wild species using intron sequences and microsatellite markers.Annals of botany · 2013Article
- Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources.PloS one · 2013Article
- Article
- Genetic diversity analysis of common beans based on molecular markers.Genetics and molecular biology · 2011Article
- Microsatellite based analysis of genetic diversity of popular black pepper genotypes in South India.Genetica · 2011Article
- Genetic structure and relationships within and between cultivated and wild sorghum (Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- The SSR-based molecular profile of 1005 grapevine (Vitis vinifera L.) accessions uncovers new synonymy and parentages, and reveals a large admixture amongst varieties of different geographic origin.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Genetic structure, diversity, and allelic richness in composite collection and reference set in chickpea (Cicer arietinum L.).BMC plant biology · 2008Article
- Merging microsatellite data: enhanced methodology and software to combine genotype data for linkage and association analysis.BMC bioinformatics · 2008Article
- Microsatellite based genetic diversity and relationships among ten Creole and commercial cattle breeds raised in Brazil.BMC genetics · 2007Article
- A cautionary tale: Lack of consistency in allele sizes between two laboratories for a published multilocus microsatellite typing system.Journal of clinical microbiology · 2007Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput fluorescent genotyping requires a considerable amount of automation for accurate and efficient processing of genetic markers. Automated DNA sequencers and corresponding software products are commercially available that contribute substantially to increased throughput rates for large-scale genotyping projects. However, some conceptually simple tasks still require time-consuming manual intervention that imposes bottlenecks on throughput capacity. One of these tasks is the conversion of imprecise DNA fragment sizes determined by commercial software programs to the underlying discrete alleles that the sizes represent. Here we describe a simple method for assigning allele sizes into their appropriate allele "bins" using least-squares minimization procedures. The method requires no special treatment of family data on plates, internal/external size standards, or electropherogram data manipulation. Tests of the method using the ABI 373A automated DNA sequencer and accompanying Genescan/Genotyper software resulted in accurate automatic classification of all alleles in >80% of 208 markers analyzed, with the remaining 20% being appropriately identified as requiring additional attention to laboratory conditions. Specific characteristics of different markers, including differences in PCR product size and inexact repeat lengths (e.g., 1. 9 bp for a dinucleotide repeat), are accommodated by the method and their properties discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.