ArticleJournal of virology1997
Rotaviruses induce an early membrane permeabilization of MA104 cells and do not require a low intracellular Ca2+ concentration to initiate their replication cycle.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Article
- Most rotavirus strains require the cation-independent mannose-6-phosphate receptor, sortilin-1, and cathepsins to enter cells.Virus research · 2018Article
- Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus.Gene · 2017Article
- Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model.Molecular medicine reports · 2016Article
- CDC42 Use in Viral Cell Entry Processes by RNA Viruses.Viruses · 2015Review
- Rotaviruses reach late endosomes and require the cation-dependent mannose-6-phosphate receptor and the activity of cathepsin proteases to enter the cell.Journal of virology · 2014Article
- Characterization of human astrovirus cell entry.Journal of virology · 2014Article
- Cross-linking of rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry.Journal of virology · 2011Article
- Rhesus rotavirus entry into a polarized epithelium is endocytosis dependent and involves sequential VP4 conformational changes.Journal of virology · 2011Article
- Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosis.Journal of virology · 2010Article
- Encapsulated cargo internalized by fusogenic liposomes partially overlaps the endoplasmic reticulum.Journal of cellular and molecular medicine · 2009Article
- A protein kinase A-dependent mechanism by which rotavirus affects the distribution and mRNA level of the functional tight junction-associated protein, occludin, in human differentiated intestinal Caco-2 cells.Journal of virology · 2007Article
- Entry of Rice dwarf virus into cultured cells of its insect vector involves clathrin-mediated endocytosis.Journal of virology · 2007Article
- Rotavirus spike protein VP4 binds to and remodels actin bundles of the epithelial brush border into actin bodies.Journal of virology · 2006Article
- The peptide-binding and ATPase domains of recombinant hsc70 are required to interact with rotavirus and reduce its infectivity.Journal of virology · 2006Article
- Dissecting rotavirus particle-raft interaction with small interfering RNAs: insights into rotavirus transit through the secretory pathway.Journal of virology · 2006Article
- Adenovirus protein VI mediates membrane disruption following capsid disassembly.Journal of virology · 2005Article
- Characterization of rotavirus cell entry.Journal of virology · 2004Article
- Discrete domains within the rotavirus VP5* direct peripheral membrane association and membrane permeability.Journal of virology · 2004Article
- Requirement for vacuolar H+ -ATPase activity and Ca2+ gradient during entry of rotavirus into MA104 cells.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, we found that rotavirus infection induces an early membrane permeabilization of MA104 cells and promotes the coentry of toxins, such as alpha-sarcin, into the cell. This cell permeability was shown to depend on infectious virus and was also shown to be virus dose dependent, with 10 infectious particles per cell being sufficient to achieve maximum permeability; transient, lasting no more than 15 min after virus entry and probably occurring concomitantly with virus penetration; and specific, since cells that are poorly permissive for rotavirus were not permeabilized. The rotavirus-mediated coentry of toxins was not blocked by the endocytosis inhibitors dansylcadaverine and cytochalasin D or by the vacuolar proton-ATPase inhibitor bafilomycin A1, suggesting that neither endocytocis nor an intraendosomal acidic pH or a proton gradient is required for permeabilization of the cells. Compounds that raise the intracellular concentration of calcium ([Ca2+]i) by different mechanisms, such as the calcium ionophores A23187 and ionomycin and the endoplasmic reticulum calcium-ATPase inhibitor thapsigargin, did not block the coentry of alpha-sarcin or affect the onset of viral protein synthesis, suggesting that a low [Ca2+]i is not essential for the initial steps of the virus life cycle. Since the entry of alpha-sarcin correlates with virus penetration in all parameters tested, the assay for permeabilization to toxins might be a useful tool for studying and characterizing the route of entry and the mechanism used by rotaviruses to traverse the cell membrane and initiate a productive replication cycle.
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