ArticleAntimicrobial agents and chemotherapy1997
Heat-induced superaggregation of amphotericin B reduces its in vitro toxicity: a new way to improve its therapeutic index.
Article in Antimicrobial agents and chemotherapy, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Unpacking amphotericin B toxicity: a critical review of amphotericin B aggregation and its link to toxicity and antifungal activity.Archives of toxicology · 2026Review
- Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity.Analytical chemistry · 2025Article
- Article
- Liposomal Amphotericin B for Treatment of Leishmaniasis: From the Identification of Critical Physicochemical Attributes to the Design of Effective Topical and Oral Formulations.Pharmaceutics · 2022Review
- Amphotericin B release rate is the link between drug status in the liposomal bilayer and toxicity.Asian journal of pharmaceutical sciences · 2022Article
- Self-assembled nanostructures of L-ascorbic acid alkyl esters support monomeric amphotericin B.Heliyon · 2021Article
- Lipid Systems for the Delivery of Amphotericin B in Antifungal Therapy.Pharmaceutics · 2020Review
- Article
- Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals.Scientific reports · 2017Article
- Influence of the freeze-drying process on the physicochemical and biological properties of pre-heated amphotericin B micellar systems.AAPS PharmSciTech · 2014Article
- Significance of algal polymer in designing amphotericin B nanoparticles.TheScientificWorldJournal · 2014Article
- NanoDisk containing super aggregated amphotericin B: a high therapeutic index antifungal formulation with enhanced potency.International journal of nanomedicine · 2013Article
- How can micelle systems be rebuilt by a heating process?International journal of nanomedicine · 2012Article
- Amphotericin B-induced renal tubular cell injury is mediated by Na+ Influx through ion-permeable pores and subsequent activation of mitogen-activated protein kinases and elevation of intracellular Ca2+ concentration.Antimicrobial agents and chemotherapy · 2009Article
- Optimizing efficacy of Amphotericin B through nanomodification.International journal of nanomedicine · 2007Review
- Potent plasmodicidal activity of a heat-induced reformulation of deoxycholate-amphotericin B (Fungizone) against Plasmodium falciparum.Antimicrobial agents and chemotherapy · 2005Article
- Treatment of experimental visceral leishmaniasis with amphotericin B in stable albumin microspheres.Antimicrobial agents and chemotherapy · 2004Article
- Heat-treated Fungizone retains amphotericin B antifungal activity without renal toxicity in rats infected with Aspergillus fumigatus.Pharmaceutical research · 2004Article
- Effect of heat-treated amphotericin B on renal and fungal cytotoxicity.Antimicrobial agents and chemotherapy · 2004Article
- The effect of serum albumin on amphotericin B aggregate structure and activity.Pharmaceutical research · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superaggregation of amphotericin B (AmB) was previously shown to occur upon heating of solutions at 70 degrees C. In the present study, we demonstrate that heat pretreatment of Fungizone (deoxycholate salt of AmB [AmB-DOC]) solutions induces a drastic decrease in the in vitro toxicity of this antibiotic. Heated AmB-DOC colloidal solutions, which mainly contained superaggregated and monomeric forms of the antibiotic, were strongly less hemolytic than unheated solutions (aggregates and monomers). Thermal pretreatment of AmB-DOC solutions also reduced the toxicity to the cell line HT29, as deduced from two simultaneous cell viability assays (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase release). These heated colloidal solutions were only slightly less efficient than the unheated ones at inhibiting the growth of Candida albicans cells in vitro. Such results suggest that mild heat treatment of AmB-DOC solutions could provide a new and simple solution for improving the therapeutic index of this antifungal agent by reducing its toxicity to mammalian cells.
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