ArticleBiophysical journal1997
Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Regulation of Pore Evolution via Progressive Electroporation Enhanced Intracellular Molecule Transport.Research (Washington, D.C.) · 2026Article
- Pulsed Field Ablation: A Review of Preclinical and Clinical Studies.Bioengineering (Basel, Switzerland) · 2025Review
- Electroporation in Head-and-Neck Cancer: An Innovative Approach with Immunotherapy and Nanotechnology Combination.Cancers · 2022Review
- Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro.Molecules (Basel, Switzerland) · 2021Article
- Peculiarities of Neurostimulation by Intense Nanosecond Pulsed Electric Fields: How to Avoid Firing in Peripheral Nerve Fibers.International journal of molecular sciences · 2021Article
- The application of PEF technology in food processing and human nutrition.Journal of food science and technology · 2021Review
- The interplay of excitation and electroporation in nanosecond pulse stimulation.Bioelectrochemistry (Amsterdam, Netherlands) · 2020Review
- Pulsed Electric Fields Can Create Pores in the Voltage Sensors of Voltage-Gated Ion Channels.Biophysical journal · 2020Article
- Extracellular-CaPharmaceutics · 2020Article
- A Review on Electroporation-Based Intracellular Delivery.Molecules (Basel, Switzerland) · 2018Review
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Control by Low Levels of Calcium of Mammalian Cell Membrane Electropermeabilization.The Journal of membrane biology · 2018Article
- Asymmetric Patterns of Small Molecule Transport After Nanosecond and Microsecond Electropermeabilization.The Journal of membrane biology · 2018Article
- Enhanced intracellular delivery via coordinated acoustically driven shear mechanoporation and electrophoretic insertion.Scientific reports · 2018Article
- Transport of charged small molecules after electropermeabilization - drift and diffusion.BMC biophysics · 2018Article
- Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation.Scientific reports · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.European biophysics journal : EBJ · 2015Review
- Cell-cell proximity effects in multi-cell electroporation.Biomicrofluidics · 2014Article
- Evidence for electro-induced membrane defects assessed by lateral mobility measurement of a GPi anchored protein.European biophysics journal : EBJ · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interaction of two stains (propidium iodide and ethidium bromide) with electropermeabilized living Chinese hamster ovary cells is observed using an ultrafast fluorescence image acquisition system. The computing process is linked to an ultra-low-light intensifying camera working with a very short time resolution (3.33 ms per image). Altered parts of the cell membrane were identified via the enhancement in fluorescence intensity of the dyes. They reflect the electropermeabilized part of the membrane in which free flow of dye occurred. Images of the fluorescence interaction patterns of the two dyes, in a maximum 20-ms time lag after pulsation, reveal asymmetrical permeabilization of the cell membrane. For electric field intensities higher than a first threshold value, permeabilization is always observed on the anode-facing side of the cell. For electric field intensities over a second higher threshold value, the two electrode-facing hemispheres of the cell are permeabilized, the hemisphere facing the anode being most permeable. These data support the conclusion that electropermeabilization of living cell membrane is affected by its resting potential. The asymmetrical pattern of the dye interaction is not dependent on the nature or concentration of the dye, the ionic strength of the pulsing buffer, or the duration of the pulse. The field intensity determines the fraction of the membrane in which molecular alterations can occur. The extent of alteration in this localized region is determined by the duration of the pulse when a single pulse in the millisecond time range is applied.
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