ArticleGenes & development1997
The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 122 citations in OpenAlex.
- Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast.Nature communications · 2023Article
- Regulation of Cdc42 protein turnover modulates the filamentous growth MAPK pathway.The Journal of cell biology · 2022Article
- Generation and characterization of conditional yeast mutants affecting each of the 2 essential functions of the scaffolding proteins Boi1/2 and Bem1.G3 (Bethesda, Md.) · 2022Article
- Cdc42-Specific GTPase-Activating Protein Rga1 Squelches Crosstalk between the High-Osmolarity Glycerol (HOG) and Mating Pheromone Response MAPK Pathways.Biomolecules · 2021Article
- Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation.Cell reports · 2021Article
- Article
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway inJournal of cell science · 2020Article
- Cell-cycle control of cell polarity in yeast.The Journal of cell biology · 2019Article
- The Cdc42 effectors Gic1 and Gic2 regulate polarized post-Golgi secretion.Cell & bioscience · 2019Article
- The shared role of the Rsr1 GTPase and Gic1/Gic2 in Cdc42 polarization.Molecular biology of the cell · 2018Article
- Establishment of the PAR-1 cortical gradient by the aPKC-PRBH circuit.Nature chemical biology · 2018Article
- Article
- Evolutionary dynamics in the fungal polarization network, a mechanistic perspective.Biophysical reviews · 2017Review
- Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2.Genetics · 2016Article
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Prioritized Expression of BTN2 of Saccharomyces cerevisiae under Pronounced Translation Repression Induced by Severe Ethanol Stress.Frontiers in microbiology · 2016Article
- ModuleRole: a tool for modulization, role determination and visualization in protein-protein interaction networks.PloS one · 2014Article
- The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation.eLife · 2013Article
- Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p.Molecular biology of the cell · 2012Article
15 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
BEM2 of Saccharomyces cerevisiae encodes a Rho-type GTPase-activating protein that is required for proper bud site selection at 26 degrees C and for bud emergence at elevated temperatures. We show here that the temperature-sensitive growth phenotype of bem2 mutant cells can be suppressed by increased dosage of the GIC1 gene. The Gic1 protein, together with its structural homolog Gic2, are required for cell size and shape control, bud site selection, bud emergence, actin cytoskeletal organization, mitotic spindle orientation/positioning, and mating projection formation in response to mating pheromone. Each protein contains a CRIB (Cdc42/Rac-interactive binding) motif and each interacts in the two-hybrid assay with the GTP-bound form of the Rho-type Cdc42 GTPase, a key regulator of polarized growth in yeast. The CRIB motif of Gic1 and the effector domain of Cdc42 are required for this association. Genetic experiments indicate that Gic1 and Gic2 play positive roles in the Cdc42 signal transduction pathway, probably as effectors of Cdc42. Subcellular localization studies with a functional green fluorescent protein-Gic1 fusion protein indicate that this protein is concentrated at the incipient bud site of unbudded cells, at the bud tip and mother-bud neck of budded cells, and at cortical sites on large-budded cells that may delimit future bud sites in the two progeny cells. The ability of Gic1 to associate with Cdc42 is important for its function but is apparently not essential for its subcellular localization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.