ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1997
Cell cycle changes in A-type lamin associations detected in human dermal fibroblasts using monoclonal antibodies.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Review
- The lamin A/C Ig-fold undergoes cell density-dependent changes that alter epitope binding.Nucleus (Austin, Tex.) · 2023Article
- Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.Life science alliance · 2023Article
- Assessment of fibroblast nuclear morphology aids interpretation of LMNA variants.European journal of human genetics : EJHG · 2019Article
- Article
- The R439C mutation in LMNA causes lamin oligomerization and susceptibility to oxidative stress.Journal of cellular and molecular medicine · 2009Article
- Lamin A/C is a risk biomarker in colorectal cancer.PloS one · 2008Article
- A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane.The Journal of cell biology · 2007Article
- Nucleoplasmic LAP2alpha-lamin A complexes are required to maintain a proliferative state in human fibroblasts.The Journal of cell biology · 2007Article
- Intramural delivery of Sirolimus prevents vascular remodeling following balloon injury.Biochimica et biophysica acta · 2007Article
- Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription.The Journal of cell biology · 2002Article
- Lamin A/C binding protein LAP2alpha is required for nuclear anchorage of retinoblastoma protein.Molecular biology of the cell · 2002Article
- Expression of individual lamins in basal cell carcinomas of the skin.British journal of cancer · 2001Article
- Head and/or CaaX domain deletions of lamin proteins disrupt preformed lamin A and C but not lamin B structure in mammalian cells.Molecular biology of the cell · 2000Article
- Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs.The EMBO journal · 2000Article
- The role of Lamin A in cytoskeleton organization in colorectal cancer cells: a proteomic investigation.Nucleus (Austin, Tex.)Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A new panel of anti-A-type lamin monoclonal antibodies was generated. Epitope mapping was performed by immunoblotting against GST-lamin fusion peptides. Epitopes were mapped to four different regions of human lamin A and three different regions of human lamin C. The distribution of A-type lamins was compared with the distribution of the proliferation marker Ki67 in proliferating and quiescent cultures of human dermal fibroblasts (HDFs) using a double indirect immunofluorescence assay. Antibodies that had been mapped to a region of the lamin C tail stained the nuclear envelope of proliferating and quiescent cells equally brightly. In contrast, antibodies recognizing epitopes in the head domain and rod domain of lamins A and C and the tail domain of lamin A stained the nuclear envelope of quiescent cells strongly but reacted poorly or not at all with the nuclear envelope of proliferating cells. Changes in the level of expression of lamins A and C were not detected in immunoblotting assays. However, epitope masking was revealed, and this occurred by two distinct mechanisms. Epitope masking in the head domain of lamins A and C occurred as a result of protein phosphorylation. Epitope masking in the rod domain of lamins A and C and in the tail domain of lamin A occurred through a physical association between the lamin and chromatin and/or other nuclear proteins. The cell cycle timing of epitope masking was investigated in HDFs that had been restimulated after serum starvation. Extensive epitope masking in restimulated cells only occurred after cells had passed through mitosis. These results are consistent with the hypothesis that rearrangement of A-type lamin filaments, as cells progress from a quiescent to a proliferating state, results in altered lamina associations.
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