ArticleThe Journal of cell biology1997
XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Spatial variation of microtubule depolymerization in large asters.Molecular biology of the cell · 2021Article
- Expression of the SEPT9_i4 isoform confers resistance to microtubule-interacting drugs.Cellular oncology (Dordrecht, Netherlands) · 2012Article
- The elegans of spindle assembly.Cellular and molecular life sciences : CMLS · 2010Review
- The 90-kDa heat shock protein Hsp90 protects tubulin against thermal denaturation.The Journal of biological chemistry · 2010Article
- Ran is required before metaphase for spindle assembly and chromosome alignment and after metaphase for chromosome segregation and spindle midbody organization.Molecular biology of the cell · 2006Article
- Protein kinase C activation promotes microtubule advance in neuronal growth cones by increasing average microtubule growth lifetimes.The Journal of cell biology · 2001Article
- XMAP215 regulates microtubule dynamics through two distinct domains.The EMBO journal · 2001Article
- TPX2, A novel xenopus MAP involved in spindle pole organization.The Journal of cell biology · 2000Article
- Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster.The Journal of cell biology · 2000Article
- D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo.The EMBO journal · 2000Article
- Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis.Molecular biology of the cell · 1999Article
- Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein.The Journal of cell biology · 1998Article
- ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.The Journal of cell biology · 1998Article
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To understand the role of microtubule-associated proteins (MAPs) in the regulation of microtubule (MT) dynamics we have characterized MAPs prepared from Xenopus laevis eggs (Andersen, S.S.L., B. Buendia, J.E. Domínguez, A. Sawyer, and E. Karsenti. 1994. J. Cell Biol. 127:1289-1299). Here we report on the purification and characterization of a 310-kD MAP (XMAP310) that localizes to the nucleus in interphase and to mitotic spindle MTs in mitosis. XMAP310 is present in eggs, oocytes, a Xenopus tissue culture cell line, testis, and brain. We have purified XMAP310 to homogeneity from egg extracts. The purified protein cross-links pure MTs. Analysis of the effect of this protein on MT dynamics by time-lapse video microscopy has shown that it increases the rescue frequency 5-10-fold and decreases the shrinkage rate twofold. It has no effect on the growth rate or the catastrophe frequency. Microsequencing data suggest that XMAP230 and XMAP310 are novel MAPs. Although the three Xenopus MAPs characterized so far, XMAP215 (Vasquez, R.J., D.L. Gard, and L. Cassimeris. 1994. J. Cell Biol. 127:985-993), XMAP230, and XMAP310 are localized to the mitotic spindle, they have distinct effects on MT dynamics. While XMAP215 promotes rapid MT growth, XMAP230 decreases the catastrophe frequency and XMAP310 increases the rescue frequency. This may have important implications for the regulation of MT dynamics during spindle morphogenesis and chromosome segregation.
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