ArticleThe Journal of cell biology1997
Targeting of the synaptic vesicle protein synaptobrevin in the axon of cultured hippocampal neurons: evidence for two distinct sorting steps.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- The synaptic vesicle cluster as a controller of pre- and postsynaptic structure and function.The Journal of physiology · 2025Review
- Article
- Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1.Molecular biology of the cell · 2015Article
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- The Alzheimer's β-secretase BACE1 localizes to normal presynaptic terminals and to dystrophic presynaptic terminals surrounding amyloid plaques.Acta neuropathologica · 2013Article
- Article
- Cyclin-dependent kinase 5 regulates the polarized trafficking of neuropeptide-containing dense-core vesicles in Caenorhabditis elegans motor neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- A heterogeneous "resting" pool of synaptic vesicles that is dynamically interchanged across boutons in mammalian CNS synapses.Brain cell biology · 2008Article
- Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2.The Biochemical journal · 2007Article
- Polarized targeting of ion channels in neurons.Pflugers Archiv : European journal of physiology · 2007Review
- Factors regulating the abundance and localization of synaptobrevin in the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Targeting mechanisms of high voltage-activated Ca2+ channels.Journal of bioenergetics and biomembranes · 2003Review
- Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles.Molecular biology of the cell · 2003Article
- Signals involved in targeting membrane proteins to synaptic vesicles.Cellular and molecular neurobiology · 2002Review
- Alternative splicing unmasks dendritic and axonal targeting signals in metabotropic glutamate receptor 1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Identification of an axonal determinant in the C-terminus of the sodium channel Na(v)1.2.The EMBO journal · 2001Article
- A conserved mechanism of synaptogyrin localization.Molecular biology of the cell · 2001Article
- Clinical mutations in the L1 neural cell adhesion molecule affect cell-surface expression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Regulation of neuronal function by protein trafficking: a role for the endosomal pathway.The Journal of physiology · 2000Review
- Di-leucine signals mediate targeting of tyrosinase and synaptotagmin to synaptic-like microvesicles within PC12 cells.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Synaptic vesicles are concentrated in the distal axon, far from the site of protein synthesis. Integral membrane proteins destined for this organelle must therefore make complex targeting decisions. Short amino acid sequences have been shown to act as targeting signals directing proteins to a variety of intracellular locations. To identify synaptic vesicle targeting sequences and to follow the path that proteins travel en route to the synaptic vesicle, we have used a defective herpes virus amplicon expression system to study the targeting of a synaptobrevin-transferrin receptor (SB-TfR) chimera in cultured hippocampal neurons. Addition of the cytoplasmic domain of synaptobrevin onto human transferrin receptor was sufficient to retarget the transferrin receptor from the dendrites to presynaptic sites in the axon. At the synapse, the SB-TfR chimera did not localize to synaptic vesicles, but was instead found in an organelle with biochemical and functional characteristics of an endosome. The chimera recycled in parallel with synaptic vesicle proteins demonstrating that the nerve terminal efficiently sorts transmembrane proteins into different pathways. The synaptobrevin sequence that controls targeting to the presynaptic endosome was not localized to a single, 10- amino acid region of the molecule, indicating that this targeting signal may be encoded by a more distributed structural conformation. However, the chimera could be shifted to synaptic vesicles by deletion of amino acids 61-70 in synaptobrevin, suggesting that separate signals encode the localization of synaptobrevin to the synapse and to the synaptic vesicle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.