ArticleThe Journal of cell biology1997
Differential localization of vesicular acetylcholine and monoamine transporters in PC12 cells but not CHO cells.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 88 citations in OpenAlex.
- Structural determinants specific for retromer protein sorting nexin 5 in regulating subcellular retrograde membrane trafficking.Journal of biomedical research · 2023Article
- SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.The Journal of cell biology · 2022Article
- Molecular, Structural, Functional, and Pharmacological Sites for Vesicular Glutamate Transporter Regulation.Molecular neurobiology · 2020Review
- Acetylcholine signaling system in progression of lung cancers.Pharmacology & therapeutics · 2019Review
- Local protein dynamics during microvesicle exocytosis in neuroendocrine cells.Molecular biology of the cell · 2018Article
- Retrograde trafficking of VMAT2 and its role in protein stability in non-neuronal cells.Journal of biomedical research · 2016Article
- Age-related attenuation of parasympathetic control of the heart in mice.International journal of physiology, pathophysiology and pharmacology · 2015Article
- Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Non-neuronal cholinergic machinery present in cardiomyocytes offsets hypertrophic signals.Journal of molecular and cellular cardiology · 2012Article
- New roles of carboxypeptidase E in endocrine and neural function and cancer.Endocrine reviews · 2012Review
- Projections from the rat pedunculopontine and laterodorsal tegmental nuclei to the anterior thalamus and ventral tegmental area arise from largely separate populations of neurons.Brain structure & function · 2011Article
- Alpha cells secrete acetylcholine as a non-neuronal paracrine signal priming beta cell function in humans.Nature medicine · 2011Article
- A distinct trans-Golgi network subcompartment for sorting of synaptic and granule proteins in neurons and neuroendocrine cells.Journal of cell science · 2011Article
- Search for the acetylcholine and vesamicol binding sites in vesicular acetylcholine transporter: the region around the lumenal end of the transport channel.Journal of neurochemistry · 2010Article
- A tyrosine-based motif localizes a Drosophila vesicular transporter to synaptic vesicles in vivo.The Journal of biological chemistry · 2010Article
- Storage and secretion of beta-NAD, ATP and dopamine in NGF-differentiated rat pheochromocytoma PC12 cells.The European journal of neuroscience · 2009Article
- Article
- Trafficking of vesicular neurotransmitter transporters.Traffic (Copenhagen, Denmark) · 2008Review
- TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Regulation of large dense-core vesicle volume and neurotransmitter content mediated by adaptor protein 3.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have indicated that neuro-endocrine cells store monoamines and acetylcholine (ACh) in different secretory vesicles, suggesting that the transport proteins responsible for packaging these neurotransmitters sort to distinct vesicular compartments. Molecular cloning has recently demonstrated that the vesicular transporters for monoamines and ACh show strong sequence similarity, and studies of the vesicular monoamine transporters (VMATs) indicate preferential localization to large dense core vesicles (LDCVs) rather than synaptic-like microvesicles (SLMVs) in rat pheochromocytoma PC12 cells. We now report the localization of the closely related vesicular ACh transporter (VAChT). In PC12 cells, VAChT differs from the VMATs by immunofluorescence and fractionates almost exclusively to SLMVs and endosomes by equilibrium sedimentation. Immunoisolation further demonstrates colocalization with synaptophysin on SLMVs as well as other compartments. However, small amounts of VAChT also occur on LDCVs. Thus, VAChT differs in localization from the VMATs, which sort predominantly to LDCVs. In addition, we demonstrate ACh transport activity in stable PC12 transformants overexpressing VAChT. Since previous work has suggested that VAChT expression confers little if any transport activity in non-neural cells, we also determined its localization in transfected CHO fibroblasts. In CHO cells, VAChT localizes to the same endosomal compartment as the VMATs by immunofluorescence, density gradient fractionation, and immunoisolation with an antibody to the transferrin receptor. We have also detected ACh transport activity in the transfected CHO cells, indicating that localization to SLMVs is not required for function. In summary, VAChT differs in localization from the VMATs in PC12 cells but not CHO cells.
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