ArticleThe Journal of cell biology1997
Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Ran modulates allosteric crosstalk between importin β surfaces.Nature communications · 2025Article
- The Nuclear Translocation of Heme Oxygenase-1 in Human Diseases.Frontiers in cell and developmental biology · 2022Review
- Methods to separate nuclear soluble fractions reflecting localizations in living cells.iScience · 2021Article
- The coming-of-age of nucleocytoplasmic transport in motor neuron disease and neurodegeneration.Cellular and molecular life sciences : CMLS · 2019Review
- Heme Oxygenases: Cellular Multifunctional and Protective Molecules against UV-Induced Oxidative Stress.Oxidative medicine and cellular longevity · 2019Review
- Enhanced diffusion by binding to the crosslinks of a polymer gel.Nature communications · 2018Article
- Importin α: functions as a nuclear transport factor and beyond.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Retinoblastoma-binding Protein 4-regulated Classical Nuclear Transport Is Involved in Cellular Senescence.The Journal of biological chemistry · 2015Article
- Nanoscale mechanism of molecular transport through the nuclear pore complex as studied by scanning electrochemical microscopy.Journal of the American Chemical Society · 2013Article
- Nucleocytoplasmic transport: a role for nonspecific competition in karyopherin-nucleoporin interactions.Molecular & cellular proteomics : MCP · 2012Article
- Fluorescence correlation spectroscopy of intact nuclear pore complexes.Biophysical journal · 2011Article
- Identification of two functional nuclear localization signals mediating nuclear import of liver receptor homologue-1.Cellular and molecular life sciences : CMLS · 2011Article
- Charge as a selection criterion for translocation through the nuclear pore complex.PLoS computational biology · 2010Article
- Avian reovirus sigmaA localizes to the nucleolus and enters the nucleus by a nonclassical energy- and carrier-independent pathway.Journal of virology · 2009Article
- Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport.Nature structural & molecular biology · 2009Article
- Functionalization of a nanopore: the nuclear pore complex paradigm.Biochimica et biophysica acta · 2009Review
- Cargo surface hydrophobicity is sufficient to overcome the nuclear pore complex selectivity barrier.The EMBO journal · 2009Article
- The intracellular mobility of nuclear import receptors and NLS cargoes.Biophysical journal · 2009Article
- Importin-beta and the small guanosine triphosphatase Ran mediate chromosome loading of the human chromokinesin Kid.The Journal of cell biology · 2008Article
- Regulation of cancer-related gene expression by galectin-3 and the molecular mechanism of its nuclear import pathway.Cancer metastasis reviews · 2007Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A 97-kD component of nuclear pore-targeting complex (the beta-subunit of nuclear pore-targeting complex [PTAC]/importin/karyopherin) mediates the import of nuclear localization signal (NLS)-containing proteins by anchoring the NLS receptor protein (the alpha-subunit of PTAC/importin/karyopherin) to the nuclear pore complex (NPC). The import requires a small GTPase Ran, which interacts directly with the beta-subunit. The present study describes an examination of the behavior of the beta-subunit in living cells and in digitonin-permeabilized cells. In living cells, cytoplasmically injected beta-subunit rapidly migrates into the nucleus. The use of deletion mutants reveals that nuclear migration of the beta-subunit requires neither Ran- nor alpha-subunit-binding but only the NPC-binding domain of this molecule, which is also involved in NLS-mediated import. Furthermore, unlike NLS-mediated import, a dominant-negative Ran, defective in GTP-hydrolysis, did not inhibit nuclear migration of the beta-subunit. In the digitonin-permeabilized cell-free import assay, the beta-subunit transits rapidly through the NPC into the nucleus in a saturating manner in the absence of exogenous addition of soluble factors. These results show that the beta-subunit undergoes translocation at the NPC in a Ran-unassisted manner when it does not carry alpha-subunit/NLS substrate. Therefore, a requirement for Ran arises only when the beta-subunit undergoes a translocation reaction together with the alpha-subunit/NLS substrate. The results provide an insight to the yet unsolved question regarding the mechanism by which proteins are directionally transported through the NPC, and the role of Ran in this process.
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