ArticleThe EMBO journal1997
Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 110 citations in OpenAlex.
- A noncontiguous code for RNA-guided DNA recognition at the origin of CRISPR-Cas.Science (New York, N.Y.) · 2026Article
- A Noncontiguous Code for RNA-Guided DNA Recognition Preceded CRISPR.bioRxiv : the preprint server for biology · 2026Article
- Group II intron and repeat-rich red algal mitochondrial genomes demonstrate the dynamic recent history of autocatalytic RNAs.BMC biology · 2022Article
- Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome.Frontiers in molecular biosciences · 2022Article
- A new RNA-DNA interaction required for integration of group II intron retrotransposons into DNA targets.Nucleic acids research · 2021Article
- A Mechanism Leading to Changes in Copy Number Variations Affected by Transcriptional Level Might Be Involved in Evolution, Embryonic Development, Senescence, and Oncogenesis Mediated by Retrotransposons.Frontiers in cell and developmental biology · 2021Article
- Recent and Ongoing Horizontal Transfer of Mitochondrial Introns Between Two Fungal Tree Pathogens.Frontiers in microbiology · 2021Article
- A group II intron-encoded protein interacts with the cellular replicative machinery through the β-sliding clamp.Nucleic acids research · 2019Article
- A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing.Journal of molecular biology · 2018Article
- Functionality ofFrontiers in molecular biosciences · 2016Article
- Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.Microbiology spectrum · 2015Review
- Evolution of group II introns.Mobile DNA · 2015Article
- Bacterial cellular engineering by genome editing and gene silencing.International journal of molecular sciences · 2014Review
- Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis.Mobile DNA · 2014Article
- Rapid targeted gene disruption in Bacillus anthracis.BMC biotechnology · 2013Article
- A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum.PloS one · 2013Article
- The brown algae Pl.LSU/2 group II intron-encoded protein has functional reverse transcriptase and maturase activities.PloS one · 2013Article
- Group II introns: mobile ribozymes that invade DNA.Cold Spring Harbor perspectives in biology · 2011Review
- Mechanisms used for genomic proliferation by thermophilic group II introns.PLoS biology · 2010Article
- Group IIC intron mobility into attC sites involves a bulged DNA stem-loop motif.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Group II introns use intron-encoded reverse transcriptase, maturase and DNA endonuclease activities for site-specific insertion into DNA. Remarkably, the endonucleases are ribonucleoprotein complexes in which the excised intron RNA cleaves the sense strand of the recipient DNA by reverse splicing, while the intron-encoded protein cleaves the antisense strand. Here, studies with the yeast group II intron aI2 indicate that both the RNA and protein components of the endonuclease contribute to recognition of an approximately 30 bp DNA target site. Our results lead to a model in which the protein component first recognizes specific nucleotides in the most distal 5' exon region of the DNA target site (E2-21 to -11). Binding of the protein then leads to DNA unwinding, enabling the intron RNA to base pair to a 13 nucleotide DNA sequence (E2-12 to E3+1) for reverse splicing. Antisense-strand cleavage requires additional interactions of the protein with the 3' exon DNA (E3+1 to +10). Our results show how enzymes can use RNA and protein subunits cooperatively to recognize specific sequences in double-stranded DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.