ArticleThe EMBO journal1997
Both RNA editing and RNA cleavage are required for translation of tobacco chloroplast ndhD mRNA: a possible regulatory mechanism for the expression of a chloroplast operon consisting of functionally unrelated genes.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 147 citations in OpenAlex.
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- Reporting complete chloroplast genome of endangered red mulberry, useful for understanding hybridization and phylogenetic relationships.Scientific reports · 2025Article
- Comparative Chloroplast Genomes and Phylogenetic Relationships of True Mangrove SpeciesCurrent issues in molecular biology · 2025Article
- Intraspecific Differentiation ofGenes · 2024Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- Plastomes ofPlants (Basel, Switzerland) · 2023Article
- The complete chloroplast genome ofFrontiers in plant science · 2023Article
- An Overview of Pentatricopeptide Repeat (PPR) Proteins in the MossPlants (Basel, Switzerland) · 2022Review
- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Transcriptome and Comparative Chloroplast Genome Analysis ofFrontiers in genetics · 2021Article
- Light/heat effects on RNA editing in chloroplast NADH-plastoquinone oxidoreductase subunit 2 (ndhB) gene of Calotropis (Calotropis procera).Journal, genetic engineering & biotechnology · 2020Article
- Sequencing and Structural Analysis of the Complete Chloroplast Genome of the Medicinal PlantPlants (Basel, Switzerland) · 2019Article
- MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology.Plant physiology · 2019Article
- Comparative Analyses of Chloroplast Genomes of Cucurbitaceae Species: Lights into Selective Pressures and Phylogenetic Relationships.Molecules (Basel, Switzerland) · 2018Article
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids.International journal of molecular sciences · 2017Article
- Plastome organization and evolution of chloroplast genes in Cardamine species adapted to contrasting habitats.BMC genomics · 2015Article
- Processing of the 5'-UTR and existence of protein factors that regulate translation of tobacco chloroplast psbN mRNA.Plant molecular biology · 2014Article
- A precise chloroplast genome of Nelumbo nucifera (Nelumbonaceae) evaluated with Sanger, Illumina MiSeq, and PacBio RS II sequencing platforms: insight into the plastid evolution of basal eudicots.BMC plant biology · 2014Article
- Strategies for metabolic pathway engineering with multiple transgenes.Plant molecular biology · 2013Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tobacco chloroplast genes encoding a photosystem I component (psaC) and a NADH dehydrogenase subunit (ndhD) are transcribed as a dicistronic pre-mRNA which is then cleaved into short mRNAs. An RNA protection assay revealed that the cleavage occurs at multiple sites in the intercistronic region. There are two possible initiation codons in the tobacco ndhD mRNA: the upstream AUG and the AUG created by RNA editing from the in-frame ACG located 25 nt downstream. Using the chloroplast in vitro translation system, we found that translation begins only from the edited AUG. The extent of ACG to AUG editing is partial and depends on developmental and environmental conditions. In addition, the in vitro assay showed that the psaC/ndhD dicistronic mRNA is not functional and that the intercistronic cleavage is a prerequisite for both ndhD and psaC translation. Using a series of mutant mRNAs, we showed that an intramolecular interaction between an 8 nt sequence in the psaC coding region and its complementary 8 nt sequence in the 5' ndhD UTR is the negative element for translation of the dicistronic mRNA. A possible mechanism in which the differential expression of the chloroplast operon consists of functionally unrelated genes is discussed.
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