ArticleThe EMBO journal1997
Parasite and mammalian GPI biosynthetic pathways can be distinguished using synthetic substrate analogues.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 77 citations in OpenAlex.
- Glycosylphosphatidylinositol Mannosyltransferase Ⅰ Protects Chinese Giant Salamander,International journal of molecular sciences · 2022Article
- Article
- Identification of TbPBN1 in Trypanosoma brucei reveals a conserved heterodimeric architecture for glycosylphosphatidylinositol-mannosyltransferase-I.Molecular microbiology · 2022Article
- Lipid profile of Trichinella papuae muscle-stage larvae.Scientific reports · 2020Article
- Fragment screening reveals salicylic hydroxamic acid as an inhibitor of Trypanosoma brucei GPI GlcNAc-PI de-N-acetylase.Carbohydrate research · 2014Article
- Identification and functional analysis of Trypanosoma cruzi genes that encode proteins of the glycosylphosphatidylinositol biosynthetic pathway.PLoS neglected tropical diseases · 2013Article
- Drug targets in Leishmania.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2010Article
- The ethanolamine branch of the Kennedy pathway is essential in the bloodstream form of Trypanosoma brucei.Molecular microbiology · 2009Article
- Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum.Molecular biology of the cell · 2008Article
- Membrane topology and transient acylation of Toxoplasma gondii glycosylphosphatidylinositols.Eukaryotic cell · 2006Article
- Chemical validation of GPI biosynthesis as a drug target against African sleeping sickness.The EMBO journal · 2004Article
- PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor.Molecular biology of the cell · 2003Article
- Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodium falciparum.Infection and immunity · 2002Article
- Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors.The EMBO journal · 2001Article
- PIG-M transfers the first mannose to glycosylphosphatidylinositol on the lumenal side of the ER.The EMBO journal · 2001Article
- Emerging themes in medicinal glycoscience.Nature biotechnology · 2000Review
- Leishmania mexicana mutants lacking glycosylphosphatidylinositol (GPI):protein transamidase provide insights into the biosynthesis and functions of GPI-anchored proteins.Molecular biology of the cell · 2000Article
- Biosynthesis of glycosylphosphatidylinositols of Plasmodium falciparum in a cell-free incubation system: inositol acylation is needed for mannosylation of glycosylphosphatidylinositols.The Biochemical journal · 1999Article
- Evidence that free GPI glycolipids are essential for growth of Leishmania mexicana.The EMBO journal · 1999Article
- Inositol acylation of glycosylphosphatidylinositols in the pathogenic fungus Cryptococcus neoformans and the model yeast Saccharomyces cerevisiae.The Biochemical journal · 1999Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Glycosylphosphatidylinositol (GPI) structures are attached to many cell surface glycoproteins in lower and higher eukaryotes. GPI structures are particularly abundant in trypanosomatid parasites where they can be found attached to complex phosphosaccharides, as well as to glycoproteins, and as mature surface glycolipids. The high density of GPI structures at all life-cycle stages of African trypanosomes and Leishmania suggests that the GPI biosynthetic pathway might be a reasonable target for the development of anti-parasite drugs. In this paper we show that synthetic analogues of early GPI intermediates having the 2-hydroxyl group of the D-myo-inositol residue methylated are recognized and mannosylated by the GPI biosynthetic pathways of Trypanosoma brucei and Leishmania major but not by that of human (HeLa) cells. These findings suggest that the discovery and development of specific inhibitors of parasite GPI biosynthesis are attainable goals. Moreover, they demonstrate that inositol acylation is required for mannosylation in the HeLa cell GPI biosynthetic pathway, whereas it is required for ethanolamine phosphate addition in the T.brucei GPI biosynthetic pathway.
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