ArticleMolecular biology of the cell1997
Electron tomography of metaphase nucleolar organizer regions: evidence for a twisted-loop organization.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Relationships between clusters of interchromatin granules and chromatin fibers.Histochemistry and cell biology · 2026Article
- Fragile sites, chromosomal lesions, tandem repeats, and disease.Frontiers in genetics · 2022Review
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Ribosomal DNA and the nucleolus in the context of genome organization.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex.Molecular biology reports · 2018Article
- The Multiple Functions of the Nucleolus in Plant Development, Disease and Stress Responses.Frontiers in plant science · 2018Review
- Article
- Nucleolar organizer regions: genomic 'dark matter' requiring illumination.Genes & development · 2016Review
- Functional ultrastructure of the plant nucleolus.Protoplasma · 2014Review
- Article
- Construction of synthetic nucleoli in human cells reveals how a major functional nuclear domain is formed and propagated through cell division.Genes & development · 2014Article
- Construction of synthetic nucleoli and what it tells us about propagation of sub-nuclear domains through cell division.Cell cycle (Georgetown, Tex.) · 2014Article
- Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function.PloS one · 2014Article
- A model for the topology of active ribosomal RNA genes.EMBO reports · 2011Article
- Localization of Nopp140 within mammalian cells during interphase and mitosis.Histochemistry and cell biology · 2009Article
- Chromatin: linking structure and function in the nucleolus.Chromosoma · 2009Review
- UBF levels determine the number of active ribosomal RNA genes in mammals.The Journal of cell biology · 2008Article
- Asymmetrical distribution of the transcriptionally competent NORs in mitosis.Journal of structural biology · 2008Article
- UBF-binding site arrays form pseudo-NORs and sequester the RNA polymerase I transcription machinery.Genes & development · 2005Article
- Three-dimensional organization of pKi-67: a comparative fluorescence and electron tomography study using FluoroNanogold.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metaphase nucleolar organizer regions (NORs), one of four types of chromosome bands, are located on human acrocentric chromosomes. They contain r-chromatin, i.e., ribosomal genes complexed with proteins such as upstream binding factor and RNA polymerase I, which are argyrophilic NOR proteins. Immunocytochemical and cytochemical labelings of these proteins were used to reveal r-chromatin in situ and to investigate its spatial organization within NORs by confocal microscopy and by electron tomography. For each labeling, confocal microscopy revealed small and large double-spotted NORs and crescent-shaped NORs. Their internal three-dimensional (3D) organization was studied by using electron tomography on specifically silver-stained NORs. The 3D reconstructions allow us to conclude that the argyrophilic NOR proteins are grouped as a fiber of 60-80 nm in diameter that constitutes either one part of a turn or two or three turns of a helix within small and large double-spotted NORs, respectively. Within crescent-shaped NORs, virtual slices reveal that the fiber constitutes several longitudinally twisted loops, grouped as two helical 250- to 300-nm coils, each centered on a nonargyrophilic axis of condensed chromatin. We propose a model of the 3D organization of r-chromatin within elongated NORs, in which loops are twisted and bent to constitute one basic chromatid coil.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.