ArticleThe Biochemical journal1997
Multiple muscle-specific regulatory elements are associated with a DNase I hypersensitive site of the cardiac beta-myosin heavy-chain gene.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- CaMKII and PKA-dependent phosphorylation co-regulate nuclear localization of HDAC4 in adult cardiomyocytes.Basic research in cardiology · 2021Article
- Novel mutations of the SRF gene in Chinese sporadic conotruncal heart defect patients.BMC medical genetics · 2020Article
- The tbx/bHLH transcription factor mga regulates gata4 and organogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Gata5 and Gata6 are functionally redundant in zebrafish for specification of cardiomyocytes.Developmental biology · 2007Article
- Factors controlling cardiac myosin-isoform shift during hypertrophy and heart failure.Journal of molecular and cellular cardiology · 2007Review
- Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality.Molecular and cellular biology · 2004Article
- Four isoforms of serum response factor that increase or inhibit smooth-muscle-specific promoter activity.The Biochemical journal · 2000Article
- Chromatin remodelling of the cardiac beta-myosin heavy chain gene.The Biochemical journal · 1998Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using nuclei isolated from neonatal cardiomyocytes, we have mapped the DNase I hypersensitive sites (DHSs) residing within the 5'-upstream regions of the hamster cardiac myosin heavy-chain (MyHC) gene. Two cardiac-specific DHSs within the 5 kb upstream region of the cardiac MyHC gene were identified. One of the DHSs was mapped to the -2.3 kb (beta-2.3 kb) region and the other to the proximal promoter region. We further localized the beta-2.3 kb site to a range of 250 bp. Multiple, conserved, muscle regulatory motifs were found within the beta-2.3 kb site, consisting of three E-boxes, one AP-2 site, one CArG motif, one CT/ACCC box and one myocyte-specific enhancer factor-2 site. This cluster of regulatory elements is strikingly similar to a cluster found in the enhancer of the mouse muscle creatine kinase gene (-1256 to -1050). The specific interaction of the motifs within the beta-2.3 kb site and the cardiac nuclear proteins was demonstrated using gel mobility-shift assays and footprinting analysis. In addition, transfection analysis revealed a significant increase in chloramphenicol acetyltransferase activity when the beta-2.3 kb site was linked to a heterologous promoter. These results suggest that previously undefined regulatory elements of the beta-MyHC gene may be associated with the beta-2.3 kb site.
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