ArticleThe Biochemical journal1997
Proteoglycan synthesis in haematopoietic cells: isolation and characterization of heparan sulphate proteoglycans expressed by the bone-marrow stromal cell line MS-5.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- The extracellular matrix of hematopoietic stem cell niches.Advanced drug delivery reviews · 2022Review
- Review
- Syndecan receptors: pericellular regulators in development and inflammatory disease.Open biology · 2021Review
- Heparan sulfate proteoglycans as key regulators of the mesenchymal niche of hematopoietic stem cells.Glycoconjugate journal · 2017Review
- Cell contractility arising from topography and shear flow determines human mesenchymal stem cell fate.Scientific reports · 2016Article
- Enhanced osteogenesis in cocultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds.Tissue engineering. Part A · 2013Article
- Glypican-1 mediates both prion protein lipid raft association and disease isoform formation.PLoS pathogens · 2009Article
- Heparan sulfate proteoglycans: a GAGgle of skeletal-hematopoietic regulators.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Review
- MMP-2 is required for bone marrow stromal cell support of pro-B-cell chemotaxis.Experimental hematology · 2005Article
- Expression of glypican-4 in haematopoietic-progenitor and bone-marrow-stromal cells.The Biochemical journal · 1999Article
- Expression of proteoglycan core proteins in human bone marrow stroma.The Biochemical journal · 1999Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteoglycans of bone-marrow stromal cells and their extracellular matrix are important components of the haematopoietic microenvironment. Recently, several studies have indicated that they are involved in the interaction of haematopoietic stem and stromal cells. However, a detailed characterization of the heparan sulphate proteoglycans synthesized by bone-marrow stromal cells is still lacking. Here we report on the isolation and characterization of proteoglycans from the haematopoietic stromal cell line MS-5, that efficiently supports the growth and differentiation of human and murine haematopoietic progenitor cells. Biochemical characterization of purified proteoglycans revealed that the haematopoietic stromal cell line MS-5 synthesizes, in addition to chondroitin sulphate proteoglycans, several different heparan sulphate proteoglycans. Immunochemical analysis, using specific antibodies against the different members of the syndecan family, glypican, betaglycan and perlecan, showed that MS-5 cells synthesize all these different heparan sulphate proteoglycans. These data were further supported by reverse-transcriptase PCR and confirmed by sequence and Northern blot analysis. The relative abundance of the different heparan sulphate proteoglycans was estimated on the protein and mRNA levels.
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