ArticleNucleic acids research1997
Adaptor-tagged competitive PCR: a novel method for measuring relative gene expression.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Glycogene Expression Profiling of Hepatic Cells by RNA-Seq Analysis for Glyco-Biomarker Identification.Frontiers in oncology · 2020Article
- High-fidelity target sequencing of individual molecules identified using barcode sequences: de novo detection and absolute quantitation of mutations in plasma cell-free DNA from cancer patients.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- Towards application of one-class classification methods to medical data.TheScientificWorldJournal · 2014Article
- Conversion of a molecular classifier obtained by gene expression profiling into a classifier based on real-time PCR: a prognosis predictor for gliomas.BMC medical genomics · 2010Article
- Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs.BMC genomics · 2008Article
- RPN2 gene confers docetaxel resistance in breast cancer.Nature medicine · 2008Article
- A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction.BMC genomics · 2007Article
- Predictive factors for response to docetaxel in human breast cancers.Cancer science · 2006Review
- A multi-class predictor based on a probabilistic model: application to gene expression profiling-based diagnosis of thyroid tumors.BMC genomics · 2006Article
- Cancer gene expression database (CGED): a database for gene expression profiling with accompanying clinical information of human cancer tissues.Nucleic acids research · 2005Article
- Identification of expressed genes linked to malignancy of human colorectal carcinoma by parametric clustering of quantitative expression data.Genome biology · 2003Article
- Multiplex polymerase chain reaction (PCR) with color-tagged module-shuffling primers for comparing gene expression levels in various cells.Nucleic acids research · 2001Article
- Identification and characterization of the potential promoter regions of 1031 kinds of human genes.Genome research · 2001Article
- Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling.Genome research · 1999Article
- Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.Nucleic acids research · 1998Article
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1 author.
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Abstract
A simple and reliable PCR-based method to quantitate gene expression is described. Following the digestion of double-stranded cDNA by a restriction enzyme, an adaptor is ligated to a cDNA from a first RNA sample, and another adaptor to a second RNA sample. The two adaptors share a common sequence at the outer region, but differ in size. Equal amounts of the ligated samples are mixed, and amplified by an adaptor-primer and a primer specific to the gene of interest. Products derived from the two sources differ in size, and can be separated by denaturing polyacrylamide gel electrophoresis. The ratio of the two products reveals the relative level of gene expression. Since the technique avoids the need to construct internal standards, it is especially useful for the analysis of many different gene transcripts.
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