ArticleNucleic acids research1997
Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 99 citations in OpenAlex.
- Microbial Metabolites-induced Epigenetic Modifications for Inhibition of Colorectal Cancer: Current Status and Future Perspectives.Mini reviews in medicinal chemistry · 2025Review
- Structural insights into DNMT5-mediated ATP-dependent high-fidelity epigenome maintenance.Molecular cell · 2022Article
- Dnmt1 has de novo activity targeted to transposable elements.Nature structural & molecular biology · 2021Article
- Epigenetic regulation in human cancer: the potential role of epi-drug in cancer therapy.Molecular cancer · 2020Review
- Downregulation of miR-152 contributes to DNMT1-mediated silencing of SOCS3/SHP-1 in non-Hodgkin lymphoma.Cancer gene therapy · 2019Article
- Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality.Frontiers in genetics · 2019Article
- Global delay in nascent strand DNA methylation.Nature structural & molecular biology · 2018Article
- Hypoxia inducible factors regulate the transcription of the sprouty2 gene and expression of the sprouty2 protein.PloS one · 2017Article
- Crystal Structure of Human DNA Methyltransferase 1.Journal of molecular biology · 2015Article
- Determining cell division symmetry through the dissection of dividing cells using single-cell expression analysis.Nature protocols · 2014Article
- Dysregulated transcriptional and post-translational control of DNA methyltransferases in cancer.Cell & bioscience · 2014Review
- RFTS-deleted DNMT1 enhances tumorigenicity with focal hypermethylation and global hypomethylation.Cell cycle (Georgetown, Tex.) · 2014Article
- Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies.Nucleic acids research · 2013Article
- Article
- Cooperative DNA and histone binding by Uhrf2 links the two major repressive epigenetic pathways.Journal of cellular biochemistry · 2011Article
- DNA methyltransferase DNMT3b protein overexpression as a prognostic factor in patients with diffuse large B-cell lymphomas.Cancer science · 2010Article
- The multi-domain protein Np95 connects DNA methylation and histone modification.Nucleic acids research · 2010Article
- MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation.Nucleic acids research · 2007Article
- The de novo methylation activity of Dnmt3a is distinctly different than that of Dnmt1.BMC biochemistry · 2005Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The original cDNA sequence reported for the murine DNA methyltransferase (MTase) was not full length. Recently, additional cDNA sequences have been reported that lie upstream of the original and contain an extended open reading frame with three additional ATGs in frame with the coding region [Tucker et al . (1996) Proc. Natl. Acad. Sci. USA , 93, 12920-12925; Yoder et al . (1996) J. Biol. Chem . 271, 31092-31097]. Genomic DNA upstream of this ATG contains two more ATGs in frame and no obvious splice site. We have constructed, and expressed in baculovirus, MTase clones that begin at each of these four ATGs and examined their properties. Constructs beginning with any of the first three ATGs as their initiator methionines give a predominant DNA MTase band of approximately 185 kDa on SDS-PAGE corresponding to translational initiation at the third ATG. The fourth ATG construct gives a much smaller protein band of 173 kDa. The 185 kDa protein was purified by HPLC, characterized by mass spectrometry and has a measured molecular mass of 184 +/- 0.5 kDa. All of these MTases were functional in vitro and steady state kinetic analysis showed that the recombinant proteins exhibit similar kinetic properties irrespective of their length. The homogeneous recombinant enzyme from the fourth ATG construct shows a 2.5-fold preference for a hemi-methylated DNA substrate as compared to an unmethylated substrate, whereas the 185 kDa protein is equally active on both substrates. The kinetic properties of the recombinant enzyme are similar to those reported for the native MTase derived from murine erythroleukemia cells. The new clones are capable of yielding large quantities of intact MTases for further structural and functional studies.
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