ArticleNucleic acids research1997
Both the polypyrimidine tract and the 3' splice site function prior to the first step of splicing in fission yeast.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 25 citations in OpenAlex.
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- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- Characterization and in vivo functional analysis of the Schizosaccharomyces pombe ICLN gene.Molecular and cellular biology · 2014Article
- Intronic sequence elements impede exon ligation and trigger a discard pathway that yields functional telomerase RNA in fission yeast.Genes & development · 2013Article
- Factors affecting splicing strength of yeast genes.Comparative and functional genomics · 2011Article
- Specific splicing defects in S. pombe carrying a degron allele of the Survival of Motor Neuron gene.The EMBO journal · 2010Article
- Large-scale comparative analysis of splicing signals and their corresponding splicing factors in eukaryotes.Genome research · 2008Article
- Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.Molecular biology of the cell · 2005Article
- Exonic splicing enhancers in fission yeast: functional conservation demonstrates an early evolutionary origin.Genes & development · 2005Article
- Interactions between two fission yeast serine/arginine-rich proteins and their modulation by phosphorylation.The Biochemical journal · 2002Article
- Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23).The EMBO journal · 2002Article
- Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.RNA (New York, N.Y.) · 2001Article
- Activation of a cryptic 5' splice site by U1 snRNA.RNA (New York, N.Y.) · 2001Article
- Evidence for splice site pairing via intron definition in Schizosaccharomyces pombe.Molecular and cellular biology · 2000Article
- Splicing factor SF1 from Drosophila and Caenorhabditis: presence of an N-terminal RS domain and requirement for viability.RNA (New York, N.Y.) · 1999Article
- Molecular genetic analysis of U2AF59 in Schizosaccharomyces pombe: differential sensitivity of introns to mutational inactivation.RNA (New York, N.Y.) · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
While it is known that several trans -acting splicing factors are highly conserved between Schizosaccharomyces pombe and mammals, the roles of cis -acting signals have received comparatively little attention. In Saccharomyces cerevisiae, sequences downstream from the branch point are not required prior to the first transesterification reaction, whereas in mammals the polypyrimidine tract and, in some introns, the 3' AG dinucleotide are critical for initial recognition of an intron. We have investigated the contribution of these two sequence elements to splicing in S.pombe. To determine the stage at which the polypyrimidine tract functions, we analyzed the second intron of the cdc2 gene (cdc 2-Int2), in which pyrimidines span the entire interval between the branch point and 3' splice site. Our data indicate that substitution of a polypurine tract results in accumulation of linear pre-mRNA, while expanding the polypyrimidine tract enhances splicing efficiency, as in mammals. To examine the role of the AG dinucleotide in cdc 2-Int2 splicing, we mutated the 3' splice junction in both the wild-type and pyrimidine tract variant RNAs. These changes block the first transesterification reaction, as in a subset of mammalian introns. However, in contrast to the situation in mammals, we were unable to rescue the first step of splicing in a 3' splice site mutant by expanding the polypyrimidine tract. Mutating the terminal G in the third intron of the nda 3 gene (nda 3-Int3) also blocks the first transesterification reaction, suggesting that early recognition of the 3' splice site is a general property of fission yeast introns. Counter to earlier work with an artificial intron, it is not possible to restore the first step of splicing in cdc 2-Int2 and nda 3-Int3 3' splice site mutants by introducing compensatory changes in U1 snRNA. These results highlight the diversity and probable redundancy of mechanisms for identifying the 3' ends of introns.
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