ArticleNucleic acids research1997
Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
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Who cites it
118 citing papers in PubMed, 287 citations in OpenAlex.
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- CRISPR/Cas9-induced DNA breaks trigger crossover, chromosomal loss, and chromothripsis-like rearrangements.The Plant cell · 2023Article
- The Influence of Edaphic Factors on DNA Damage and Repair in Wild WheatInternational journal of molecular sciences · 2023Article
- High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells.Frontiers in genome editing · 2023Article
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- Microparticle-mediated CRISPR DNA delivery for genome editing in poplar.Frontiers in plant science · 2023Article
- IntraspecificNAR genomics and bioinformatics · 2022Article
- Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering.Plant physiology · 2022Article
- Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants.Frontiers in genome editing · 2022Review
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- Raising Climate-Resilient Crops: Journey From the Conventional Breeding to New Breeding Approaches.Current genomics · 2021Review
- Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin.International journal of molecular sciences · 2021Article
- Different DNA repair pathways are involved in single-strand break-induced genomic changes in plants.The Plant cell · 2021Article
58 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double strand DNA breaks in plants are primarily repaired via non-homologous end joining. However, little is known about the molecular events underlying this process. We have studied non-homologous end joining of linearized plasmid DNA with different termini configurations following transformation into tobacco cells. A variety of sequences were found at novel end junctions. Joining with no sequence alterations was rare. In most cases, deletions were found at both ends, and rejoining usually occurred at short repeats. A distinct feature of plant junctions was the presence of relatively large, up to 1.2 kb long, insertions (filler DNA), in approximately 30% of the analyzed clones. The filler DNA originated either from internal regions of the plasmid or from tobacco genomic DNA. Some insertions had a complex structure consisting of several reshuffled plasmid-related regions. These data suggest that double strand break repair in plants involves extensive end degradation, DNA synthesis following invasion of ectopic templates and multiple template switches. Such a mechanism is reminiscent of the synthesis-dependent recombination in bacteriophage T4. It can also explain the frequent 'DNA scrambling' associated with illegitimate recombination in plants.
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