ArticleNucleic acids research1997
Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 188 citations in OpenAlex.
- Adaptive laboratory evolution of Micrococcus luteus and identification of genes associated with radioresistance through genome-wide association study.Scientific reports · 2025Article
- Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.Frontiers in molecular biosciences · 2022Article
- Characterization of the complete mitochondrial genome ofSaudi journal of biological sciences · 2021Article
- The Coevolution of Fungal Mitochondrial Introns and Their Homing Endonucleases (GIY-YIG and LAGLIDADG).Genome biology and evolution · 2020Article
- Article
- Proteomic Mapping of Multifunctional Complexes Within Triatomine Saliva.Frontiers in cellular and infection microbiology · 2020Article
- Modifying a covarying protein-DNA interaction changes substrate preference of a site-specific endonuclease.Nucleic acids research · 2019Article
- Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases.Nucleic acids research · 2018Article
- Lnc-ISG20 Inhibits Influenza A Virus Replication by Enhancing ISG20 Expression.Journal of virology · 2018Article
- An Evolutionary Perspective on Yeast Mating-Type Switching.Genetics · 2017Review
- Loss and Gain of Group I Introns in the MitochondrialZoological studies · 2017Article
- Comparative genomic analysis of the flagellin glycosylation island of the Gram-positive thermophile Geobacillus.BMC genomics · 2016Article
- Genome-wide investigation and expression analyses of the pentatricopeptide repeat protein gene family in foxtail millet.BMC genomics · 2016Article
- Article
- Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease.PloS one · 2016Article
- Control of catalytic efficiency by a coevolving network of catalytic and noncatalytic residues.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Evidence for inter-specific recombination among the mitochondrial genomes of Fusarium species in the Gibberella fujikuroi complex.BMC genomics · 2013Article
- Bacillus subtilis hlpB encodes a conserved stand-alone HNH nuclease-like protein that is essential for viability unless the hlpB deletion is accompanied by the deletion of genes encoding the AddAB DNA repair complex.Journal of bacteriology · 2012Article
- ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response.Genes & development · 2012Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The LAGLIDADG and HNH families of site-specific DNA endonucleases encoded by viruses, bacteriophages as well as archaeal, eucaryotic nuclear and organellar genomes are characterized by the sequence motifs 'LAGLIDADG' and 'HNH', respectively. These endonucleases have been shown to occur in different environments: LAGLIDADG endonucleases are found in inteins, archaeal and group I introns and as free standing open reading frames (ORFs); HNH endonucleases occur in group I and group II introns and as ORFs. Here, statistical models (hidden Markov models, HMMs) that encompass both the conserved motifs and more variable regions of these families have been created and employed to characterize known and potential new family members. A number of new, putative LAGLIDADG and HNH endonucleases have been identified including an intein-encoded HNH sequence. Analysis of an HMM-generated multiple alignment of 130 LAGLIDADG family members and the three-dimensional structure of the I- Cre I endonuclease has enabled definition of the core elements of the repeated domain (approximately 90 residues) that is present in this family of proteins. A conserved negatively charged residue is proposed to be involved in catalysis. Phylogenetic analysis of the two families indicates a lack of exchange of endonucleases between different mobile elements (environments) and between hosts from different phylogenetic kingdoms. However, there does appear to have been considerable exchange of endonuclease domains amongst elements of the same type. Such events are suggested to be important for the formation of elements of new specficity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.