ArticleNucleic acids research1997
Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance.Nature communications · 2020Article
- Antimelanoma CTL recognizes peptides derived from an ORF transcribed from the antisense strand of the 3' untranslated region of TRIT1.Molecular therapy oncolytics · 2015Article
- Analysis of wheat SAGE tags reveals evidence for widespread antisense transcription.BMC genomics · 2008Article
- Highly expressed genes are associated with inverse antisense transcription in mouse.Journal of genetics · 2007Article
- Distinct expression patterns of natural antisense transcripts in Arabidopsis.Plant physiology · 2007Article
- Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods.BMC genomics · 2006Article
- In silico discovery of human natural antisense transcripts.BMC bioinformatics · 2006Article
- Prediction of trans-antisense transcripts in Arabidopsis thaliana.Genome biology · 2006Article
- SC35 promotes sustainable stress-induced alternative splicing of neuronal acetylcholinesterase mRNA.Molecular psychiatry · 2005Article
- Genome-wide prediction and identification of cis-natural antisense transcripts in Arabidopsis thaliana.Genome biology · 2005Article
- The gene structure and expression of human ABHD1: overlapping polyadenylation signal sequence with Sec12.BMC genomics · 2003Article
- Antisense transcripts with rice full-length cDNAs.Genome biology · 2003Article
- Article
- Computational discovery of sense-antisense transcription in the human and mouse genomes.Genome biology · 2002Article
- A novel transforming growth factor beta2 antisense transcript in mammalian lung.The Biochemical journal · 1998Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The PR264/SC35 splicing factor belongs to the family of SR proteins which function as essential and alternative splicing factors. Here, we report that the human PR264/SC35 locus is bidirectionally transcribed. Double in situ hybridization experiments have allowed simultaneous detection of sense and antisense RNA in human CCRF-CEM cells, suggesting that expression of the corresponding genes is not mutually exclusive. We have characterized three main classes of ET RNAs encoded by the opposite strand of the PR264/SC35 gene and containing PR264/SC35-overlapping sequences, PR264/SC35-non overlapping sequences or a combination of both. We show that their expression results from the use of alternative promoters, exons and polyadenylation signals. PR264/SC35-non overlapping ET mRNA species potentially encode two protein isoforms (449 and 397 amino acids) and are expressed from the PR264/SC35 promoting region. Northern blots and RNase protection analyses indicate that ET polyadenylated RNAs are differentially expressed in several human cell lines. Similar studies performed in the mouse have revealed that the bidirectional transcription of the PR264/SC35 locus is a conserved mechanism and that the open reading frame identified in a subset of human ET mRNAs is highly conserved (93% homology). Northern blot analyses performed with several murine tissues confirmed the differential expression of the ET gene and revealed that it is predominantly expressed in the testis.
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