ArticleNucleic acids research1997
New dye-labeled terminators for improved DNA sequencing patterns.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
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- Human metapneumovirus prevalence and patterns of subgroup persistence identified through surveillance of pediatric pneumonia hospital admissions in coastal Kenya, 2007-2016.BMC infectious diseases · 2019Article
- Revealing Nucleic Acid Mutations Using Förster Resonance Energy Transfer-Based Probes.Sensors (Basel, Switzerland) · 2016Review
- Next-Generation Sequencing Approaches in Cancer: Where Have They Brought Us and Where Will They Take Us?Cancers · 2015Review
- Candidate gene association mapping of Sclerotinia stalk rot resistance in sunflower (Helianthus annuus L.) uncovers the importance of COI1 homologs.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- Opportunities and challenges associated with clinical diagnostic genome sequencing: a report of the Association for Molecular Pathology.The Journal of molecular diagnostics : JMD · 2012Article
- Rheonix CARD(®) Technology: An Innovative and Fully Automated Molecular Diagnostic Device.Point of care · 2012Article
- Factors affecting the detection and quantification of mitochondrial point heteroplasmy using Sanger sequencing and SNaPshot minisequencing.International journal of legal medicine · 2011Article
- An integrated system for DNA sequencing by synthesis using novel nucleotide analogues.Accounts of chemical research · 2010Article
- Pathogenicity of a highly exopolysaccharide-producing Halomonas strain causing epizootics in larval cultures of the Chilean scallop Argopecten purpuratus (Lamarck, 1819).Microbial ecology · 2009Article
- Four-color DNA sequencing with 3'-O-modified nucleotide reversible terminators and chemically cleavable fluorescent dideoxynucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The complete nucleotide sequences of the five genetically distinct plastid genomes of Oenothera, subsection Oenothera: I. sequence evaluation and plastome evolution.Nucleic acids research · 2008Article
- Association between the estrogen receptor alpha A908G mutation and outcomes in invasive breast cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2007Article
- Complete nucleotide sequence of the 113-kilobase linear catabolic plasmid pAL1 of Arthrobacter nitroguajacolicus Rü61a and transcriptional analysis of genes involved in quinaldine degradation.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used two new dye sets for automated dye-labeled terminator DNA sequencing. One set consists of four, 4,7-dichlororhodamine dyes (d-rhodamines). The second set consists of energy-transfer dyes that use the 5-carboxy-d-rhodamine dyes as acceptor dyes and the 5- or 6-carboxy isomers of 4'-aminomethylfluorescein as the donor dye. Both dye sets utilize a new linker between the dye and the nucleotide, and both provide more even peak heights in terminator sequencing than the dye-terminators consisting of unsubstituted rhodamine dyes. The unsubstituted rhodamine terminators produced electropherograms in which weak G peaks are observed after A peaks and occasionally C peaks. The number of weak G peaks has been reduced or eliminated with the new dye terminators. The general improvement in peak evenness improves accuracy for the automated base-calling software. The improved signal-to-noise ratio of the energy-transfer dye-labeled terminators combined with more even peak heights results in successful sequencing of high molecular weight DNA templates such as bacterial artificial chromosome DNA.
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