ArticleProceedings of the National Academy of Sciences of the United States of America1997
A method that allows the assembly of kinetochore components onto chromosomes condensed in clarified Xenopus egg extracts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Reconstitution of genome remodeling usingFrontiers in cell and developmental biology · 2026Review
- CCDC69 maintains genome integrity by regulating KIF2C/MCAK depolymerase activity and the stability of the chromosomal passenger complex.Scientific reports · 2024Article
- Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.Molecular biology of the cell · 2021Article
- Identification and characterization of centromeric sequences inGenome research · 2021Article
- Mechanisms that prevent catastrophic interactions between paternal chromosomes and the oocyte meiotic spindle.Cell cycle (Georgetown, Tex.) · 2018Review
- A versatile multivariate image analysis pipeline reveals features of Xenopus extract spindles.The Journal of cell biology · 2016Article
- Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Intranuclear DNA density affects chromosome condensation in metazoans.Molecular biology of the cell · 2013Article
- A cell-free system for functional centromere and kinetochore assembly.Nature protocols · 2012Article
- CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assembly.The Journal of cell biology · 2011Article
- Microtubule assembly in meiotic extract requires glycogen.Molecular biology of the cell · 2011Article
- Dissection of CENP-C-directed centromere and kinetochore assembly.Molecular biology of the cell · 2009Article
- Ectopic expression of plasma membrane targeted subunits of the Ndc80-complex as a tool to study kinetochore biochemistry.Molecular oncology · 2009Article
- Bod1, a novel kinetochore protein required for chromosome biorientation.The Journal of cell biology · 2007Article
- Article
- Measuring the stoichiometry and physical interactions between components elucidates the architecture of the vertebrate kinetochore.Molecular biology of the cell · 2005Article
- Cytoplasmic dynein is required for the nuclear attachment and migration of centrosomes during mitosis in Drosophila.The Journal of cell biology · 1999Article
- Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles.The Journal of cell biology · 1998Article
- The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function.Progress in molecular and subcellular biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Kinetochores are complex macromolecular structures that link mitotic chromosomes to spindle microtubules. Although a small number of kinetochore components have been identified, including the kinesins CENP-E and XKCM1 as well as cytoplasmic dynein, neither how these and other proteins are organized to produce a kinetochore nor their exact functions within this structure are understood. For this reason, we have developed an assay that allows kinetochore components to assemble onto discrete foci on in vitro-condensed chromosomes. The source of the kinetochore components is a clarified cell extract from Xenopus eggs that can be fractionated or immunodepleted of individual proteins. Kinetochore assembly in these clarified extracts requires preincubating the substrate sperm nuclei in an extract under low ATP conditions. Immunodepletion of XKCM1 from the extracts prevents the localization of kinetochore-associated XKCM1 without affecting the targeting of CENP-E and cytoplasmic dynein or the binding of monomeric tubulin to the kinetochore. Extension of this assay for the analysis of other components should help to dissect the protein-protein interactions involved in kinetochore assembly and function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.