ArticleProceedings of the National Academy of Sciences of the United States of America1997
A general method to design dominant negatives to B-HLHZip proteins that abolish DNA binding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Targeted Interference with USF2 Binding to the SERPINE1 Proximal Promoter E-Box in Dual Mutant p53Biomedicines · 2026Review
- Taking the Myc out of cancer: toward therapeutic strategies to directly inhibit c-Myc.Molecular cancer · 2021Review
- Selective antagonism of cJun for cancer therapy.Journal of experimental & clinical cancer research : CR · 2020Review
- Optogenetic Inhibitor of the Transcription Factor CREB.Chemistry & biology · 2015Article
- The basic helix-loop-helix/leucine zipper transcription factor USF2 integrates serum-induced PAI-1 expression and keratinocyte growth.Journal of cellular biochemistry · 2014Article
- Carboxylation of cytosine (5caC) in the CG dinucleotide in the E-box motif (CGCAG|GTG) increases binding of the Tcf3|Ascl1 helix-loop-helix heterodimer 10-fold.Biochemical and biophysical research communications · 2014Article
- The AP-1 binding sites located in the pol gene intragenic regulatory region of HIV-1 are important for viral replication.PloS one · 2011Article
- Ectopic Mitf in the embryonic chick retina by co-transfection of β-catenin and Otx2.Investigative ophthalmology & visual science · 2010Article
- Max-E47, a designed minimalist protein that targets the E-box DNA site in vivo and in vitro.Journal of the American Chemical Society · 2009Article
- Protein sequestration generates a flexible ultrasensitive response in a genetic network.Molecular systems biology · 2009Article
- 5'UTR of the neurogenic bHLH Nex1/MATH-2/NeuroD6 gene is regulated by two distinct promoters through CRE and C/EBP binding sites.Journal of neuroscience research · 2007Article
- c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma.Genes & development · 2006Article
- Upstream stimulatory factors, USF1 and USF2, bind to the human haem oxygenase-1 proximal promoter in vivo and regulate its transcription.The Biochemical journal · 2004Article
- Evidence for a cancer-specific switch at the CDK4 promoter with loss of control by both USF and c-Myc.Oncogene · 2004Article
- The zipper region of Epstein-Barr virus bZIP transcription factor Zta is necessary but not sufficient to direct DNA binding.Journal of virology · 2003Article
- B-ZIP proteins encoded by the Drosophila genome: evaluation of potential dimerization partners.Genome research · 2002Article
- The Usf-1 transcription factor is a novel target for the stress-responsive p38 kinase and mediates UV-induced Tyrosinase expression.The EMBO journal · 2001Article
- Tissue-specific transgenic knockdown of Fos-related antigen 2 (Fra-2) expression mediated by dominant negative Fra-2.Molecular and cellular biology · 2001Article
- Article
- Cell-type-dependent activity of the ubiquitous transcription factor USF in cellular proliferation and transcriptional activation.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a method to design dominant-negative proteins (D-N) to the basic helix-loop-helix-leucine zipper (B-HLHZip) family of sequence-specific DNA binding transcription factors. The D-Ns specifically heterodimerize with the B-HLHZip dimerization domain of the transcription factors and abolish DNA binding in an equimolar competition. Thermal denaturation studies indicate that a heterodimer between a Myc B-HLHZip domain and a D-N consisting of a 12-amino acid sequence appended onto the Max dimerization domain (A-Max) is -6.3 kcal.mol-1 more stable than the Myc:Max heterodimer. One molar equivalent of A-Max can totally abolish the DNA binding activity of a Myc:Max heterodimer. This acidic extension also has been appended onto the dimerization domain of the B-HLHZip protein Mitf, a member of the transcription factor enhancer binding subfamily, to produce A-Mitf. The heterodimer between A-Mitf and the B-HLHZip domain of Mitf is -3.7 kcal.mol-1 more stable than the Mitf homodimer. Cell culture studies show that A-Mitf can inhibit Mitf-dependent transactivation both in acidic extension and in a dimerization-dependent manner. A-Max can inhibit Myc-dependent foci formation twice as well as the Max dimerization domain (HLHZip). This strategy of producing D-Ns may be applicable to other B-HLHZip or B-HLH proteins because it provides a method to inhibit the DNA binding of these transcription factors in a dimerization-specific manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.