ArticleThe Biochemical journal1997
Differential expression and regulation of ryanodine receptor and myo-inositol 1,4,5-trisphosphate receptor Ca2+ release channels in mammalian tissues and cell lines.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 65 citations in OpenAlex.
- IPAntioxidants (Basel, Switzerland) · 2024Article
- Chimeras of sperm PLCζ reveal disparate protein domain functions in the generation of intracellular Ca2+ oscillations in mammalian eggs at fertilization.Molecular human reproduction · 2013Article
- Article
- Ca2+ entry following P2X receptor activation induces IP3 receptor-mediated Ca2+ release in myocytes from small renal arteries.British journal of pharmacology · 2011Article
- Ca(2+) puffs originate from preestablished stable clusters of inositol trisphosphate receptors.Science signaling · 2009Article
- Article
- Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Bcl-2 regulator FKBP38 is activated by Ca2+/calmodulin.The EMBO journal · 2005Article
- Mechanistic and functional changes in Ca2+ entry after retinoic acid-induced differentiation of neuroblastoma cells.The Biochemical journal · 2005Article
- Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).The Journal of comparative neurology · 2004Article
- Co-incident signalling between mu-opioid and M3 muscarinic receptors at the level of Ca2+ release from intracellular stores: lack of evidence for Ins(1,4,5)P3 receptor sensitization.The Biochemical journal · 2003Article
- Ryanodine stores and calcium regulation in the inner segments of salamander rods and cones.The Journal of physiology · 2003Article
- In situ modulation of the human cardiac ryanodine receptor (hRyR2) by FKBP12.6.The Biochemical journal · 2003Article
- Nicotinic acid-adenine dinucleotide phosphate (NAADP) elicits specific microsomal Ca2+ release from mammalian cells.The Biochemical journal · 2001Article
- Lysophosphatidic acid-mediated Ca2+ mobilization in human SH-SY5Y neuroblastoma cells is independent of phosphoinositide signalling, but dependent on sphingosine kinase activation.The Biochemical journal · 1999Article
- Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.The Journal of physiology · 1999Article
- Protein-protein interactions in intracellular Ca2+-release channel function.The Biochemical journal · 1999Review
- Ca2+-induced Ca2+ release in chromaffin cells seen from inside the ER with targeted aequorin.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ryanodine receptors (RyRs) and Ins(1,4,5)P3 receptors (Ins(1,4, 5)P3Rs) represent two multigene families of channel proteins that mediate the release of Ca2+ ions from intracellular stores. In the present study, the expression patterns of these channel proteins in mammalian cell lines and tissues were investigated by using isoform-specific antibodies. All cell lines examined expressed two or more Ins(1,4,5)P3R isoforms, with the type 1 Ins(1,4,5)P3R being ubiquitous. RyR isoforms were detected in only six out of eight cell lines studied. Similarly, of the nine rabbit tissues examined, RyR protein expression was detected only in brain, heart, skeletal muscle and uterus. Specific [3H]ryanodine binding was found in a number of rabbit tissues, although it was not detected in mammalian cell lines. Subcellular fractionation of SH-SY5Y human neuroblastomas revealed that the type 2 RyR and type 1 Ins(1,4,5)P3R co-localize among the fractions of a sucrose-cushion separation of crude microsomal membrane fractions. Manipulation of SH-SY5Y cells by chronic stimulation of muscarinic acetylcholine receptor (mAChR) results in a decrease in their type 1 Ins(1,4,5)P3R levels but not in the abundance of the type 2 RyR. Differentiation of these neuroblastomas by using retinoic acid did not detectably alter their expression of Ca2+-release channel proteins. Finally, differentiation of BC3H1 cells affects the expression of their Ca2+-release channel proteins in an isoform-specific manner. In summary, this study demonstrates that mammalian cell lines display distinct patterns of Ca2+-release channel protein expression. The abundance of these proteins is differentially regulated during phenotypic modifications of a cell, such as differentiation or chronic stimulation of mAChR.
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