ArticleThe Biochemical journal1997
Mechanism of cAMP-induced Ca2+ influx in Dictyostelium: role of phospholipase A2.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Ion Signaling in Cell Motility and Development inBiomolecules · 2024Review
- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- The professional phagocyte Dictyostelium discoideum as a model host for bacterial pathogens.Current drug targets · 2011Review
- Oscillatory signaling and network responses during the development of Dictyostelium discoideum.Ageing research reviews · 2008Review
- The regulation of cell motility and chemotaxis by phospholipid signaling.Journal of cell science · 2008Review
- Arachidonic acid is a chemoattractant for Dictyostelium discoideum cells.Journal of biosciences · 2007Article
- PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis.Developmental cell · 2007Article
- The contractile vacuole in Ca2+-regulation in Dictyostelium: its essential function for cAMP-induced Ca2+-influx.BMC developmental biology · 2006Article
- Release of Ca2+ from the endoplasmic reticulum contributes to Ca2+ signaling in Dictyostelium discoideum.Eukaryotic cell · 2005Article
- Ca2+ regulation in the absence of the iplA gene product in Dictyostelium discoideum.BMC cell biology · 2005Article
- A link of Ca2+ to cAMP oscillations in Dictyostelium: the calmodulin antagonist W-7 potentiates cAMP relay and transiently inhibits the acidic Ca2+-store.BMC developmental biology · 2004Article
- Mechanism of cAMP-induced H(+)-efflux of Dictyostelium cells: a role for fatty acids.Journal of biosciences · 2000Article
- G protein beta subunit-null mutants are impaired in phagocytosis and chemotaxis due to inappropriate regulation of the actin cytoskeleton.The Journal of cell biology · 1998Article
- Fatty acids induce release of Ca2+ from acidosomal stores and activate capacitative Ca2+ entry in Dictyostelium discoideum.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cAMP-induced Ca2+ influx in Dictyostelium follows two pathways: a G-protein-dependent pathway where influx is reduced by 50-70% in Galpha2 and Gbeta-negative strains and a heterotrimeric G-protein-independent pathway. Using a pharmacological approach, we found that phospholipase A2 (PLA2) is the target of both pathways. The products of PLA2 activity, arachidonic acid (AA) and palmitic acid, induced Ca2+ influx to a similar extent as cAMP. Half-maximal activation occurred at 3 microM AA and saturation at 10 microM AA. The response to AA was quantitatively similar throughout early differentiation and thus independent of cAMP-receptor concentration. Synergy experiments revealed that cAMP and AA acted through identical pathways. The PLA2-activating peptide, a peptide with sequence similarity to the G-protein beta-subunit, activated Ca2+ influx. The G-protein-independent pathway was sensitive to genistein but not to blockers of protein kinase C and other kinases, suggesting that tyrosine kinase may directly or indirectly activate PLA2 in this case.
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