ArticleThe Biochemical journal1997
Homodimerization and hetero-oligomerization of the single-domain trefoil protein pNR-2/pS2 through cysteine 58.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 74 citations in OpenAlex.
- Characterization of Monomeric and Dimeric Forms of the Lectin TFF1 in the Human Vagina: Possible Role for the Innate Immune Defence.International journal of molecular sciences · 2026Article
- Structure, Function, and Therapeutic Potential of the Trefoil Factor Family in the Gastrointestinal Tract.ACS pharmacology & translational science · 2020Review
- Chemical synthesis of human trefoil factor 1 (TFF1) and its homodimer provides novel insights into their mechanisms of action.Chemical communications (Cambridge, England) · 2020Article
- The Tumor Suppressor TFF1 Occurs in Different Forms and Interacts with Multiple Partners in the Human Gastric Mucus Barrier: Indications for Diverse Protective Functions.International journal of molecular sciences · 2020Article
- Molecular Alterations in the Stomach ofInternational journal of molecular sciences · 2020Article
- Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1-3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis.International journal of molecular sciences · 2019Article
- The Interaction ofInternational journal of molecular sciences · 2019Review
- Binding ofMicroorganisms · 2018Article
- Urine Trefoil Factors as Prognostic Biomarkers in Chronic Kidney Disease.BioMed research international · 2018Article
- TFF3 is a valuable predictive biomarker of endocrine response in metastatic breast cancer.Endocrine-related cancer · 2015Article
- Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis.BMC biotechnology · 2015Article
- The trefoil factor interacting protein TFIZ1 binds the trefoil protein TFF1 preferentially in normal gastric mucosal cells but the co-expression of these proteins is deregulated in gastric cancer.The international journal of biochemistry & cell biology · 2009Article
- Trefoil factor 3 stimulates human and rodent pancreatic islet beta-cell replication with retention of function.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Molecular forms of trefoil factor 1 in normal gastric mucosa and its expression in normal and abnormal gastric tissues.World journal of gastroenterology · 2006Article
- Expression of trefoil factors 1 and 2 in precancerous condition and gastric cancer.World journal of gastroenterology · 2006Article
- Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor.The Journal of clinical investigation · 2005Article
- Relationship between trefoil factor 1 expression and gastric mucosa injuries and gastric cancer.World journal of gastroenterology · 2005Article
- Helicobacter pylori interacts with the human single-domain trefoil protein TFF1.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Article
- Dramatic diurnal variation in the concentration of the human trefoil peptide TFF2 in gastric juice.Gut · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The single-domain human trefoil proteins [pNR-2/pS2 and human intestinal trefoil factor (hITF)] have seven cysteine residues, of which six are involved in maintaining the structure of the trefoil domain. The seventh does not form part of the trefoil domain and is located three residues from the C-terminus. The ability of the pNR-2/pS2 single trefoil domain protein to dimerize was examined by using recombinant protein with either a cysteine or a serine residue at this position by equilibrium ultracentrifugation, laser-assisted desorption MS, gel filtration and PAGE. pNR-2/pS2 Cys58 formed dimers, whereas pNR-2/pS2 Ser58 did not. Experiments in which the dimer was treated with thiol agents demonstrated that the dimer was linked via a disulphide bond and that the intermolecular disulphide bond was more susceptible to reduction than the intramolecular disulphide bonds. To examine whether dimeric pNR-2/pS2 was secreted by oestrogen-responsive breast cancer cells, which are known to express pNR-2/pS2 mRNA, conditioned medium was separated on non-denaturing polyacrylamide gels, transferred to PVDF membrane and reacted with antiserum against pNR-2/pS2. Monomeric and dimeric pNR-2/pS2 were detected but the majority of the protein reactivity was associated with a larger protein. Treatment of this protein with thiol agents suggested that it is an oligomer containing pNR-2/pS2 linked to another protein by a disulphide bond. These studies suggest that the biological action of pNR-2/pS2 single-domain trefoil protein might involve the formation of homodimers or oligomers with other proteins.
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