ArticleGenes & development1997
A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 218 citations in OpenAlex.
- Cysteine Mutagenesis of a Group II Intron-Encoded Protein Supports Splicing, Mobility, and Site-Specific Labeling.Biochemistry · 2025Article
- Article
- Protein-free catalysis of DNA hydrolysis and self-integration by a ribozyme.Nucleic acids research · 2025Article
- Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes.Nature communications · 2024Article
- A Novel Thermostable and Processive Reverse Transcriptase from a Group II Intron ofBiomolecules · 2023Article
- Microbial gene expression in Guaymas Basin subsurface sediments responds to hydrothermal stress and energy limitation.The ISME journal · 2023Article
- Structures of a mobile intron retroelement poised to attack its structured DNA target.Science (New York, N.Y.) · 2022Article
- Widespread phages of endosymbionts: Phage WO genomics and the proposed taxonomic classification of Symbioviridae.PLoS genetics · 2022Article
- A new RNA-DNA interaction required for integration of group II intron retrotransposons into DNA targets.Nucleic acids research · 2021Article
- Organellar Introns in Fungi, Algae, and Plants.Cells · 2021Review
- Molecular characterization of both transesterification reactions of the group II intron circularization pathway.Nucleic acids research · 2021Article
- Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota.Cell · 2021Article
- The ChloroplastCells · 2021Review
- Group II intron as cold sensor for self-preservation and bacterial conjugation.Nucleic acids research · 2020Article
- Unraveling the role of the enigmatic MatK maturase in chloroplast group IIA intron excision.Plant direct · 2020Article
- Article
- Bacterial group II introns generate genetic diversity by circularization and trans-splicing from a population of intron-invaded mRNAs.PLoS genetics · 2018Article
- Structural accommodations accompanying splicing of a group II intron RNP.Nucleic acids research · 2018Article
- Article
- An ultraprocessive, accurate reverse transcriptase encoded by a metazoan group II intron.RNA (New York, N.Y.) · 2018Article
38 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
The Lactococcus lactis group II intron Ll.ltrB is similar to mobile yeast mtDNA group II introns, which encode reverse transcriptase, RNA maturase, and DNA endonuclease activities for site-specific DNA insertion. Here, we show that the Lactococcal intron can be expressed and spliced efficiently in Escherichia coli. The intron-encoded protein LtrA has reverse transcriptase and RNA maturase activities, with the latter activity shown both in vivo and in vitro, a first for any group II intron-encoded protein. As for the yeast mtDNA introns, the DNA endonuclease activity of the Lactococcal intron is associated with RNP particles containing both the intron-encoded protein and the excised intron RNA. Also, the intron RNA cleaves the sense-strand of the recipient DNA by a reverse splicing reaction, whereas the intron-encoded protein cleaves the antisense strand. The Lactococcal intron endonuclease can be obtained in large quantities by coexpression of the LtrA protein with the intron RNA in E. coli or reconstituted in vitro by incubating the expressed LtrA protein with in vitro-synthesized intron RNA. Furthermore, the specificity of the endonuclease and reverse splicing reactions can be changed predictably by modifying the RNA component. Expression in E. coli facilitates the use of group II introns for the targeting of specific foreign sequences to a desired site in DNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.