ArticleInfection and immunity1997
Phase variation and conservation of lipooligosaccharide epitopes in Haemophilus somnus.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- Characterization of proteins present in the biofilm matrix and outer membrane vesicles ofmBio · 2025Article
- Whole-genome sequencing ofVeterinary world · 2023Article
- Extraction and Electrophoretic Analysis of Bacterial Lipopolysaccharides and Outer Membrane Proteins.Bio-protocol · 2021Article
- Genome microsatellite diversity within the Apicomplexa phylum.Molecular genetics and genomics : MGG · 2016Article
- Microsatellites in Pursuit of Microbial Genome Evolution.Frontiers in microbiology · 2015Review
- Histophilus somni causes extracellular trap formation by bovine neutrophils and macrophages.Microbial pathogenesis · 2013Article
- The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle.Veterinary research · 2012Article
- Molecular characterization of phosphorylcholine expression on the lipooligosaccharide of Histophilus somni.Microbial pathogenesis · 2009Article
- Platelet activation by Histophilus somni and its lipooligosaccharide induces endothelial cell proinflammatory responses and platelet internalization.Shock (Augusta, Ga.) · 2008Article
- Identification of genes involved in the expression of atypical lipooligosaccharide structures from a second class of Haemophilus ducreyi.Infection and immunity · 2007Article
- Phase and antigenic variation in bacteria.Clinical microbiology reviews · 2004Review
- Incorporation of N-acetylneuraminic acid into Haemophilus somnus lipooligosaccharide (LOS): enhancement of resistance to serum and reduction of LOS antibody binding.Infection and immunity · 2002Article
- Haemophilus somnus induces apoptosis in bovine endothelial cells in vitro.Infection and immunity · 2001Article
- Antigenic diversity of Haemophilus somnus lipooligosaccharide: phase-variable accessibility of the phosphorylcholine epitope.Journal of clinical microbiology · 2000Article
- Molecular cloning and mutagenesis of a DNA locus involved in lipooligosaccharide biosynthesis in Haemophilus somnus.Infection and immunity · 2000Article
- Short-sequence DNA repeats in prokaryotic genomes.Microbiology and molecular biology reviews : MMBR · 1998Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The bovine-specific pathogen Haemophilus somnus is capable of undergoing structural and antigenic phase variation in its lipooligosaccharide (LOS) components after in vivo and in vitro passage. However, commensal isolates from the reproductive tract have not been observed to vary in phase (T. J. Inzana, R. P. Gogolewski, and L. B. Corbeil, Infect. Immun. 60:2943-2951, 1992). We now report that specific monoclonal antibodies (MAbs) to the LOSs of Haemophilus aegyptius, Neisseria gonorrhoeae, and Haemophilus influenzae, as well as H. somnus, reacted with some phase-variable epitopes in H. somnus LOS. All reactive MAbs bound to LOS components of about 4.3 kDa in the same H. somnus isolates, including a non-phase-varying strain. Following in vitro passage of a clonal variant of strain 738 that was nonreactive with the MAbs, 11.8% of young colonies shifted to a reactive phenotype. A digoxigenin-labelled 5'-CAATCAATCAATCAATCAATCAATCAAT-3' oligonucleotide probe hybridized to genomic DNA from strain 738 but did not react with DNA from a non-phase-varying strain. Sequence analysis of the gene containing 5'-CAAT-3' tandem sequences revealed 48% amino acid homology with the lex-2B gene-encoded protein of H. influenzae type b. Our results indicate that some LOS epitopes are conserved between H. somnus and other Haemophilus and Neisseria species, that LOS phase variation may occur at a high rate in some strains of H. somnus, and that phase variation may, in part, be due to 5'-CAAT-3' tandem sequences present in H. somnus genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.