Evidence map›Paper›PMID 9352939›Full record

ArticleJournal of bacteriology1997

Cloning of a Vibrio alginolyticus rpoN gene that is required for polar flagellar formation.

I Kawagishi, M Nakada, N Nishioka, M Homma

Abstract readComparative Study
In one paragraph

Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Polar flagellar motility of the Vibrionaceae.Microbiology and molecular biology reviews : MMBR · 2001
    Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

I KawagishiDivision of Biological Science, Graduate School of Science, Nagoya University, Japan.
M Nakada
N Nishioka
M Homma

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A fragment of DNA was cloned which complemented a polar flagellum-defective (pof) mutation of Vibrio alginolyticus. The fragment contained two complete and two partial open reading frames (ORFs) (ORF2 and -3 and ORF1 and -4, respectively). The presumed product of ORF2 has an amino acid sequence with a high degree of similarity to that of RpoN, which is an alternative sigma factor (sigma54) for other microorganisms. The other ORFs are also homologous to the genes adjacent to other rpoN genes. Deletion analysis suggests that ORF2 complements the pof mutation. These results demonstrate that RpoN is involved in the expression of polar flagellar genes.

Indexed as

DNA-Binding ProteinsGenes, BacterialAmino Acid SequenceBlotting, SouthernCloning, MolecularDNA-Directed RNA PolymerasesFlagellaGenetic Complementation TestMolecular Sequence DataRestriction MappingRNA Polymerase Sigma 54Sequence Analysis, DNASequence Homology, Amino AcidSigma FactorSpecies SpecificityVibrioDNA-Binding ProteinsDNA-Directed RNA PolymerasesRNA Polymerase Sigma 54Sigma Factor

Identifiers

PMID9352939
PMCPMC179618

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.