ArticleJournal of bacteriology1997
A tricarboxylic acid cycle intermediate regulating transcription of a chloroaromatic biodegradative pathway: fumarate-mediated repression of the clcABD operon.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Metabolomics for biomarker discovery in the diagnosis, prognosis, survival and recurrence of colorectal cancer: a systematic review.Oncotarget · 2017Pooled it
- Occurrence of BTEX from petroleum hydrocarbons in surface water, sediment, and biota from Ubeji Creek of Delta State, Nigeria.Environmental science and pollution research international · 2021Article
- Transcriptional Modulation of Transport- and Metabolism-Associated Gene Clusters Leading to Utilization of Benzoate in Preference to Glucose in Pseudomonas putida CSV86.Applied and environmental microbiology · 2017Article
- Modulation of glucose transport causes preferential utilization of aromatic compounds in Pseudomonas putida CSV86.Journal of bacteriology · 2007Article
- Preferential utilization of aromatic compounds over glucose by Pseudomonas putida CSV86.Applied and environmental microbiology · 2006Article
- Dual role of response regulator StyR in styrene catabolism regulation.Applied and environmental microbiology · 2005Article
- TouR-mediated effector-independent growth phase-dependent activation of the sigma54 Ptou promoter of Pseudomonas stutzeri OX1.Journal of bacteriology · 2004Article
- Bacterial transcriptional regulators for degradation pathways of aromatic compounds.Microbiology and molecular biology reviews : MMBR · 2004Review
- Real-time reverse transcription-PCR analysis of expression of halobenzoate and salicylate catabolism-associated operons in two strains of Pseudomonas aeruginosa.Applied and environmental microbiology · 2003Article
- Transcriptional cross-regulation of the catechol and protocatechuate branches of the beta-ketoadipate pathway contributes to carbon source-dependent expression of the Acinetobacter sp. strain ADP1 pobA gene.Applied and environmental microbiology · 2003Article
- Fumarate-mediated inhibition of erythrose reductase, a key enzyme for erythritol production by Torula corallina.Applied and environmental microbiology · 2002Article
- Inactivation of cytochrome o ubiquinol oxidase relieves catabolic repression of the Pseudomonas putida GPo1 alkane degradation pathway.Journal of bacteriology · 2002Article
- Genetic and biochemical characterization of a 2,4,6-trichlorophenol degradation pathway in Ralstonia eutropha JMP134.Journal of bacteriology · 2002Article
- Integration of global regulation of two aromatic-responsive sigma(54)-dependent systems: a common phenotype by different mechanisms.Journal of bacteriology · 2002Article
- Role of the crc gene in catabolic repression of the Pseudomonas putida GPo1 alkane degradation pathway.Journal of bacteriology · 2001Article
- The black cat/white cat principle of signal integration in bacterial promoters.The EMBO journal · 2001Review
- Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9.Journal of bacteriology · 1999Article
- The PalkBFGHJKL promoter is under carbon catabolite repression control in Pseudomonas oleovorans but not in Escherichia coli alk+ recombinants.Journal of bacteriology · 1999Article
- Carbon-source-dependent expression of the PalkB promoter from the Pseudomonas oleovorans alkane degradation pathway.Journal of bacteriology · 1998Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
The ortho-cleavage pathways of catechol and 3-chlorocatechol are central catabolic pathways of Pseudomonas putida that convert aromatic and chloroaromatic compounds to tricarboxylic acid (TCA) cycle intermediates. They are encoded by the evolutionarily related catBCA and clcABD operons, respectively. Expression of the cat and clc operons requires the LysR-type transcriptional activators CatR and ClcR, respectively, and the inducer molecules cis,cis-muconate and 2-chloro-cis,cis-muconate, respectively. The regulation of the cat and clc promoters has been well studied, but the extent to which these operons are repressed by growth in TCA cycle intermediates has not been explored. We demonstrate by transcriptional fusion studies that the expression from the clc promoter is repressed when the cells are grown on succinate, citrate, or fumarate and that this repression is ClcR dependent and occurs at the transcriptional level. The presence of these organic acids did not affect the expression from the cat promoter. In vitro transcription assays demonstrate that the TCA cycle intermediate fumarate directly and specifically inhibits the formation of the clcA transcript. No such inhibition was observed when CatR was used as the activator on either the cat or clc template. Titration studies of fumarate and 2-chloromuconate show that the fumarate effect is concentration dependent and reversible, indicating that fumarate and 2-chloromuconate most probably compete for the same binding site on ClcR. This is an interesting example of the transcriptional regulation of a biodegradative pathway by the intracellular sensing of the state of the TCA cycle.
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