ArticleThe EMBO journal1997
Functional differences between the human LINE retrotransposon and retroviral reverse transcriptases for in vivo mRNA reverse transcription.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Interferons and Cytokines Induce Transcriptional Activation of the Long-Interspersed Element-1 in Myeloid Cells from Autoimmune Patients.European journal of immunology · 2025Article
- No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing.Cell reports · 2021Article
- Cytoplasmic synthesis of endogenousProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Properties of LINE-1 proteins and repeat element expression in the context of amyotrophic lateral sclerosis.Mobile DNA · 2018Article
- L1 retrotransposition in the soma: a field jumping ahead.Mobile DNA · 2018Review
- Article
- Protein-Coding Genes' Retrocopies and Their Functions.Viruses · 2017Review
- Localized Retroprocessing as a Model of Intron Loss in the Plant Mitochondrial Genome.Genome biology and evolution · 2016Article
- Identification of L1 ORF2p sequence important to retrotransposition using Bipartile Alu retrotransposition (BAR).Nucleic acids research · 2016Article
- Novel Role of 3'UTR-Embedded Alu Elements as Facilitators of Processed Pseudogene Genesis and Host Gene Capture by Viral Genomes.PloS one · 2016Article
- Restricting retrotransposons: a review.Mobile DNA · 2016Review
- Article
- Laboratory methods for the analysis of primate mobile elements.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Human RNA "rumor" viruses: the search for novel human retroviruses in chronic disease.Microbiology and molecular biology reviews : MMBR · 2008Review
- Extensive individual variation in L1 retrotransposition capability contributes to human genetic diversity.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Retrotransposition-Competent Human LINE-1 Induces Apoptosis in Cancer Cells With Intact p53.Journal of biomedicine & biotechnology · 2004Article
- Molecular evolution of eukaryotic genomes: hemiascomycetous yeast spliceosomal introns.Nucleic acids research · 2003Article
- Identifying related L1 retrotransposons by analyzing 3' transduced sequences.Genome biology · 2003Article
- Molecular archeology of L1 insertions in the human genome.Genome biology · 2002Article
- Unusual expression of LINE-1 transposable element in the MRL autoimmune lymphoproliferative syndrome-prone strain.Oncogene · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analysed the reverse transcriptase (RT) activity of the human LINE retrotransposon and that of two retroviruses, using an in vivo assay within mammalian (murine and human) cells. The assay relies on transfection of the cells with expression vectors for the RT of the corresponding elements and PCR analysis of the DNA extracted 2-4 days post-transfection using primers bracketing the intronic domains of co-transfected reporter genes or of cellular genes. This assay revealed high levels of reverse-transcribed cDNA molecules, with the intron spliced out, with expression vectors for the LINE. Generation of cDNA molecules requires LINE ORF2, whereas ORF1 is dispensable. Deletion derivatives within the 3.8 kb LINE ORF2 allowed further delineation of the RT domain: > 0.7 kb at the 5'-end of the LINE ORF2 is dispensable for reverse transcription, consistent with this domain being an endonuclease-like domain, as well as 1 kb at the 3'-end, a putative RNase H domain. Conversely, the RT of the two retroviruses tested, Moloney murine leukemia virus and human immunodeficiency virus, failed to produce similar reverse transcripts. These experiments demonstrate a specific and high efficiency reverse transcription activity for the LINE RT, which applies to RNA with no sequence specificity, including those from cellular genes, and which might therefore be responsible for the endogenous activity that we previously detected within mammalian cells through the formation of pseudogene-like structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.