ArticleThe EMBO journal1997
Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers, 1 of them a synthesis that pooled it.
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Who cites it
125 citing papers in PubMed, 1 synthesis or guideline pooled it, 466 citations in OpenAlex.
- Pluripotent stem cells: induction and self-renewal.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Pooled it
- Antisense Oligonucleotide-MediatedAnimals : an open access journal from MDPI · 2026Article
- Cell fusion after wounding requires plasma membrane damage and endocytosis.bioRxiv : the preprint server for biology · 2026Article
- Cell Fusion in Reprogramming and Regeneration.Advances in experimental medicine and biology · 2026Review
- Intercellular mRNA transfer alters the human pluripotent stem cell state.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Inducing Pluripotency in Somatic Cells: Historical Perspective and Recent Advances.International journal of stem cells · 2024Review
- Inter-cellular mRNA Transfer Alters Human Pluripotent Stem Cell State.bioRxiv : the preprint server for biology · 2024Article
- Human iPS Cells for Clinical Applications and Cellular Products.Handbook of experimental pharmacology · 2023Article
- Cyclic DNA remethylation following active demethylation at euchromatic regions in mouse embryonic stem cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- The Master Regulator Protein BAZ2B Can Reprogram Human Hematopoietic Lineage-Committed Progenitors into a Multipotent State.Cell reports · 2020Article
- Production of interspecies somatic/pluripotent heterokaryons using polyethylene glycol (PEG) and selection by imaging flow cytometry for the study of nuclear reprogramming.Cytotechnology · 2020Article
- Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research.Stem cell research & therapy · 2020Review
- Understanding and Modulating Immunity With Cell Reprogramming.Frontiers in immunology · 2019Review
- Embryonic germ cell extracts erase imprinted genes and improve the efficiency of induced pluripotent stem cells.Scientific reports · 2018Article
- DNA methylation dynamics of genomic imprinting in mouse development.Biology of reproduction · 2018Review
- Automatic cell fusion via optically-induced dielectrophoresis and optically-induced locally-enhanced electric field on a microfluidic chip.Biomicrofluidics · 2018Article
- Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues.Scientific reports · 2017Article
- DUSP9 Modulates DNA Hypomethylation in Female Mouse Pluripotent Stem Cells.Cell stem cell · 2017Article
- Reprogramming cell fates by small molecules.Protein & cell · 2017Review
- Diet-induced adipose tissue expansion is mitigated in mice with a targeted inactivation of mesoderm specific transcript (Mest).PloS one · 2017Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Genomic reprogramming of primordial germ cells (PGCs), which includes genome-wide demethylation, prevents aberrant epigenetic modifications from being transmitted to subsequent generations. This process also ensures that homologous chromosomes first acquire an identical epigenetic status before an appropriate switch in the imprintable loci in the female and male germ lines. Embryonic germ (EG) cells have a similar epigenotype to PGCs from which they are derived. We used EG cells to investigate the mechanism of epigenetic modifications in the germ line by analysing the effects on a somatic nucleus in the EG-thymic lymphocyte hybrid cells. There were striking changes in methylation of the somatic nucleus, resulting in demethylation of several imprinted and non-imprinted genes. These epigenetic modifications were heritable and affected gene expression as judged by re-activation of the silent maternal allele of Peg1/Mest imprinted gene in the somatic nucleus. This remarkable change in the epigenotype of the somatic nucleus is consistent with the observed pluripotency of the EG-somatic hybrid cells as they differentiated into a variety of tissues in chimeric embryos. The epigenetic modifications observed in EG-somatic cell hybrids in vitro are comparable to the reprogramming events that occur during germ cell development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.